Andrés A, Satrústegui J, Machado A
Biol Neonate. 1983;43(3-4):198-204. doi: 10.1159/000241629.
The behavior of the principal NADPH-consuming pathways, fatty acid synthesis (-ATP-citrate lyase), transhydrogenase, glutamate dehydrogenase, glutathione reductase, NADPH-cytochrome c reductase and mitochondrial and cytoplasmic thioredoxin reductases were studied during the development of the heart, brain and liver of the rat. The liver is the tissue with highest NADPH-consuming activities. Transhydrogenase activity is highest in the heart; it undergoes two steep increases of activity, one at birth and another between 15 and 25 days. Liver cytoplasmic glutathione reductase activity increases mainly at birth. The activities in heart and brain are lower and the brain enzyme activity slightly increases throughout development. Liver NADPH-cytochrome c reductase activity greatly increases at weaning. The heart activity is the lowest, no changes taking place in the developmental period. Heart mitochondrial and brain cytoplasmic thioredoxin reductases undergo increases in activity at birth. The ratios of NADPH-producing/NADPH-consuming activities are constant in all tissues studied, and oscillate between congruent to 4 in fetuses to congruent to 2 in adults, the only exception being the adult heart ratio (congruent to 23). This otherwise constancy confirms that both processes are closely correlated.
在大鼠心脏、大脑和肝脏发育过程中，对主要消耗NADPH的途径，即脂肪酸合成（-ATP-柠檬酸裂解酶）、转氢酶、谷氨酸脱氢酶、谷胱甘肽还原酶、NADPH-细胞色素c还原酶以及线粒体和细胞质硫氧还蛋白还原酶的行为进行了研究。肝脏是消耗NADPH活性最高的组织。转氢酶活性在心脏中最高；其活性经历两次急剧增加，一次在出生时，另一次在15至25天之间。肝脏细胞质谷胱甘肽还原酶活性主要在出生时增加。心脏和大脑中的活性较低，且大脑中的酶活性在整个发育过程中略有增加。肝脏NADPH-细胞色素c还原酶活性在断奶时大幅增加。心脏中的活性最低，在发育期间没有变化。心脏线粒体和大脑细胞质硫氧还蛋白还原酶在出生时活性增加。在所研究的所有组织中，产生NADPH/消耗NADPH活性的比率是恒定的，在胎儿中约为4，在成体中约为2，唯一的例外是成体心脏的比率（约为23）。这种在其他方面的恒定性证实了这两个过程密切相关。
