人血小板孵育过程中环氧化酶对内源性花生四烯酸的优先利用

Preferential utilization of endogenous arachidonate by cyclo-oxygenase in incubations of human platelets.

作者信息

Sautebin L, Caruso D, Galli G, Paoletti R

出版信息

FEBS Lett. 1983 Jun 27;157(1):173-8. doi: 10.1016/0014-5793(83)81140-5.

Abstract

Thromboxane B2 (TXB2) and 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) formed from the endogenous and exogenous arachidonate during human platelet incubation, was evaluated by selected ion monitoring (SIM). TXB2 formed from endogenous substrate accounted for about one third of the total, whereas the great part of 12-HETE derived from exogenous arachidonate. These data indicate that under the tested conditions the pool of arachidonate that acts as substrate for cyclo-oxygenase is different from the pool that acts as substrate for lipoxygenase and that the arachidonate released from phospholipids is preferentially utilized by cyclo-oxygenase.

摘要

通过选择离子监测（SIM）评估了人血小板孵育过程中由内源性和外源性花生四烯酸形成的血栓素B2（TXB2）和12-羟基-5,8,10,14-二十碳四烯酸（12-HETE）。由内源性底物形成的TXB2约占总量的三分之一，而大部分12-HETE来源于外源性花生四烯酸。这些数据表明，在测试条件下，作为环氧化酶底物的花生四烯酸池与作为脂氧合酶底物的池不同，并且从磷脂释放的花生四烯酸优先被环氧化酶利用。