Stuart M J, Allen J B
Pediatrics. 1982 Jun;69(6):714-8.
An assessment of arachidonic acid metabolism in the platelet of the neonate was performed. The uptake of [14C]arachidonic acid into platelets of both the neonate and the adult were similar. Neonatal platelets, however, released a significantly greater amount (P less than .001) of prelabeled arachidonic acid (24.7% +/- 2.8%) in response to the physiologic agent thrombin when compared with platelets from adult control subjects (14.6% +/- 0.8%). When the activities of the lipoxygenase (12-L-hydroxy-5,8,10,14-eicosatetraenoic acid) and cyclooxygenase pathways (12-L-hydroxy-5,8,10-heptadecatrienoic acid and thromboxane B2) were evaluated following incubation of platelets with [14C]arachidonic acid, significant differences were observed between adult and neonatal platelets. Platelets from the neonate produced less (P less than .01) thromboxane B2 (11.1% +/- 1.7%) when compared with platelets from adult control subjects (19% +/- 1.7%). In contrast, the lipoxygenase product 12-L-hydroxy-5,8,10,14-eicostatetraenoic acid was increased (P less than .005) in the platelet from the neonate (41.5% +/- 2%), when compared with the adult (31.2% +/- 2.1%). The observation that the availability of substrate arachidonic acid is increased in the platelet of the neonate may have general implications in neonatal pathophysiologic processes.
对新生儿血小板中的花生四烯酸代谢进行了评估。[14C]花生四烯酸进入新生儿和成人血小板的摄取情况相似。然而，与成年对照受试者的血小板相比，新生儿血小板在生理介质凝血酶作用下释放出显著更多（P小于0.001）的预先标记的花生四烯酸（24.7%±2.8%）。在用[14C]花生四烯酸孵育血小板后评估脂氧合酶（12-L-羟基-5,8,10,14-二十碳四烯酸）和环氧化酶途径（12-L-羟基-5,8,10-十七碳三烯酸和血栓素B2）的活性时，观察到成年和新生儿血小板之间存在显著差异。与成年对照受试者的血小板（19%±1.7%）相比，新生儿的血小板产生的血栓素B2较少（P小于0.01）（11.1%±1.7%）。相反，与成年人（31.2%±2.1%）相比，新生儿血小板中的脂氧合酶产物12-L-羟基-5,8,10,14-二十碳四烯酸增加（P小于0.005）（41.5%±2%）。新生儿血小板中底物花生四烯酸可用性增加这一观察结果可能对新生儿病理生理过程具有普遍意义。
