Zambon J J, Slots J, Genco R J
Infect Immun. 1983 Jul;41(1):19-27. doi: 10.1128/iai.41.1.19-27.1983.
Actinobacillus actinomycetemcomitans from the human oral cavity was serologically characterized with rabbit antisera to the type strain NCTC 9710; a number of reference strains, including Y4, ATCC 29522, ATCC 29523, ATCC 29524, NCTC 9709; and our own isolates representative of each of 10 biotypes. Using immunoabsorbed antisera, we identified three distinct serotypes by immunodiffusion and indirect immunofluorescence. Serotype a was represented by ATCC 29523 and SUNYaB 75; serotype b was represented by ATCC 29522 and Y4; and serotype c was represented by NCTC 9710 and SUNYaB 67. Indirect immunofluorescence revealed no reaction between the three A. actinomycetemcomitans serotype-specific antisera and 62 strains representing 23 major oral bacterial species. Distinct from the serotype antigens were at least one A. actinomycetemcomitans species common antigen and an antigen shared with other Actinobacillus species, Haemophilus aphrophilus, and Haemophilus paraphrophilus. All serotype a A. actinomycetemcomitans strains failed to ferment xylose, whereas all serotype b organisms fermented xylose. Serotype c included xylose-positive as well as xylose-negative strains. A total of 301 isolates of A. actinomycetemcomitans from the oral cavity of 74 subjects were serologically categorized by indirect immunofluorescence with serotype-specific rabbit antisera. Each patient harbored only one serotype of A. actinomycetemcomitans. Fourteen healthy subjects, five diabetics, and seventeen adult periodontitis patients exhibited serotypes a and b in approximately equal frequency, whereas serotype c was found less frequently. In contrast, in 29 localized juvenile periodontitis patients, the incidence of serotype b was approximately two times higher than that of serotypes a or c, suggesting a particularly high periodontopathic potential of A. actinomycetemcomitans serotype b strains. In subjects infected with A. actinomycetemcomitans, serum antibodies were detected to the serotype antigens, indicating that these antigens may play a role in the pathogenesis of periodontal disease.
用人抗血清对来自人类口腔的伴放线放线杆菌进行血清学特征分析，该抗血清针对模式菌株NCTC 9710；一些参考菌株，包括Y4、ATCC 29522、ATCC 29523、ATCC 29524、NCTC 9709；以及我们自己分离的代表10种生物型的菌株。使用免疫吸附抗血清，我们通过免疫扩散和间接免疫荧光鉴定出三种不同的血清型。血清型a以ATCC 29523和SUNYaB 75为代表；血清型b以ATCC 29522和Y4为代表；血清型c以NCTC 9710和SUNYaB 67为代表。间接免疫荧光显示，三种伴放线放线杆菌血清型特异性抗血清与代表23种主要口腔细菌物种的62株菌株之间无反应。与血清型抗原不同的是，至少有一种伴放线放线杆菌属共同抗原以及一种与其他放线杆菌属、嗜沫嗜血杆菌和副嗜沫嗜血杆菌共有的抗原。所有血清型a的伴放线放线杆菌菌株均不能发酵木糖，而所有血清型b的菌株都能发酵木糖。血清型c包括木糖阳性和木糖阴性菌株。用血清型特异性兔抗血清通过间接免疫荧光对来自74名受试者口腔的301株伴放线放线杆菌分离株进行血清学分类。每位患者仅携带一种血清型的伴放线放线杆菌。14名健康受试者、5名糖尿病患者和17名成人牙周炎患者中血清型a和b的出现频率大致相等，而血清型c的出现频率较低。相比之下，在29名局限性青少年牙周炎患者中，血清型b的发生率比血清型a或c高出约两倍，这表明伴放线放线杆菌血清型b菌株具有特别高的致牙周病潜力。在感染伴放线放线杆菌的受试者中，检测到针对血清型抗原的血清抗体，表明这些抗原可能在牙周病的发病机制中起作用。
