Department of Periodontology, School of Dental Medicine, University of Connecticut, Farmington, Connecticut, USA.
Department of Oral Health Practice, Periodontology Division and Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA.
J Periodontol. 2024 Jul;95(7):650-661. doi: 10.1002/JPER.23-0504. Epub 2024 Mar 13.
The aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar-incisor pattern periodontitis (C-MIP) affecting the primary or permanent dentitions.
DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated.
C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01).
Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.
本研究旨在评估影响乳牙或恒牙的 C 型磨牙-切牙型牙周炎（C-MIP）患者的龈下微生物组。
从 45 名 C-MIP 患者的患病和健康部位的龈下生物膜样本中提取 DNA，并进行系统发育微阵列分析。比较了乳牙受影响和恒牙受影响的 C-MIP 部位。还评估了 C-MIP 受影响部位与牙位匹配的健康部位之间的个体内差异。
受乳牙影响的 C-MIP 部位与受恒牙影响的 C-MIP 部位的微生物群落部分重叠但明显不同（p<0.05）。差异是由于某些属（Capnocytophaga 和 Leptotrichia）的某些物种在乳牙 C-MIP 部位的水平增加，而 Filifactor alocis 在恒牙 C-MIP 部位更为普遍。Aggregatibacter actinomycetemcomitans（Aa）是在乳牙和恒牙 C-MIP 部位均高流行和高水平的物种之一。此外，与同一受试者的牙位匹配的健康部位相比，恒牙和乳牙 C-MIP 部位均显示出不同的微生物群落（p<0.01）。
患有 C-MIP 的乳牙和恒牙表现出一种失调的微生物组，受乳牙影响的儿童与受恒牙影响的儿童表现出明显不同的特征。然而，Aa 在乳牙和恒牙的患病部位均丰富，证实该微生物与两种牙位的 C-MIP 有关。
