Tan E L, Patel P V, Parsons N J, Martin P M, Smith H
J Gen Microbiol. 1986 May;132(5):1407-13. doi: 10.1099/00221287-132-5-1407.
On SDS-PAGE, solubilized and proteinase K treated preparations of Neisseria gonorrhoeae strain BS4 (agar) showed differences in silver stained lipopolysaccharide (LPS) patterns, before and after induction to resistance to serum killing by incubation for 3 h at 37 degrees C with low Mr fractions from lysates of guinea pig red blood cells. Preparations from the original serum susceptible gonococci and LPS purified from such bacteria showed two components, but the preparations from the serum resistant gonococci were deficient in the higher Mr component. Furthermore, on immunoblotting with fresh human serum (FHS), the two LPS components of the susceptible gonococci reacted strongly with IgM. With preparations from the serum resistant gonococci there was no reaction in the area corresponding to the higher Mr component and a weaker reaction with the component of low Mr. Purified LPS from the susceptible gonococci neutralized the bactericidal activity of FHS against N. gonorrhoeae strain BS4 (agar) probably by reacting with the relevant antibody, since heated FHS was no longer bactericidal when mixed with a source of complement (human placental serum) after prior reaction with the LPS. These neutralization tests coupled with the results of immunoblotting strongly suggest that increased serum resistance is due to the lack of the high Mr LPS moiety.
在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上，淋病奈瑟菌菌株BS4（琼脂）经溶解和蛋白酶K处理后的制剂，在与豚鼠红细胞裂解物的低分子量组分于37℃孵育3小时诱导产生血清杀伤抗性前后，银染脂多糖（LPS）模式存在差异。原始血清敏感淋球菌的制剂以及从这类细菌中纯化的LPS显示有两个组分，但血清抗性淋球菌的制剂中高分子量组分缺失。此外，用新鲜人血清（FHS）进行免疫印迹时，敏感淋球菌的两种LPS组分与IgM强烈反应。血清抗性淋球菌的制剂在对应高分子量组分的区域无反应，与低分子量组分的反应较弱。敏感淋球菌纯化的LPS可能通过与相关抗体反应中和了FHS对淋病奈瑟菌菌株BS4（琼脂）的杀菌活性，因为在与LPS预先反应后，加热的FHS与补体来源（人胎盘血清）混合时不再具有杀菌作用。这些中和试验与免疫印迹结果强烈表明，血清抗性增加是由于高分子量LPS部分的缺失。
