Shinomiya T, Shiga S, Kageyama M
Mol Gen Genet. 1983;189(3):375-81. doi: 10.1007/BF00325898.
Thirty-seven mutants defective in pyocin R2 production in the P. aeruginosa PAO strain were subjected to fine mapping of pyocin R2 genes by transduction with phage F116L. Sixteen complementation groups (designated prtA through prtP) involved in pyocin R2 production were tentatively identified by complementation tests using phage F116L. Their linkages to trpC and trpE markers and fine mapping by three point crosses demonstrated that most of the mutations (prtA through prtN) were located in between trpC and trpE, and that the prtP mutation was localized outside this major prt cluster but in the proximity of the rifA and strA region.
利用噬菌体F116L转导，对铜绿假单胞菌PAO菌株中37个产绿脓菌素R2缺陷型突变体进行绿脓菌素R2基因的精细定位。通过使用噬菌体F116L进行互补试验，初步鉴定出16个参与绿脓菌素R2产生的互补群（命名为prtA至prtP）。它们与trpC和trpE标记的连锁关系以及三点杂交的精细定位表明，大多数突变（prtA至prtN）位于trpC和trpE之间，而prtP突变位于这个主要的prt簇之外，但靠近rifA和strA区域。
