Shinomiya T, Shiga S, Kikuchi A, Kageyama M
Mol Gen Genet. 1983;189(3):382-9. doi: 10.1007/BF00325899.
The chromosome segment which contains the genes responsible for production of pyocin R2 in P. aeruginosa PAO was defined physically using R-prime plasmids constructed in vivo from R68.45. The previous conclusion from genetic mapping that the cluster of pyocin R2 genes is located in between trpC and trpE genes was confirmed by deletion mapping of various R prime plasmids bearing the trpC gene. The pyocin R2 gene cluster was further localized on two contiguous HindIII fragments of 16 kb and 8.0 kb. PML14 strain, in which R-type pyocin genes were completely deleted, had only one 11 kb HindIII fragment instead. Heteroduplexes between this 11 kb fragment with the two HindIII fragments of PAO revealed that the cluster of pyocin R2 genes was an insertion 13 kb long.
利用从R68.45体内构建的R-prime质粒，对铜绿假单胞菌PAO中负责产生绿脓菌素R2的基因所在的染色体片段进行了物理定位。先前通过遗传图谱得出的绿脓菌素R2基因簇位于trpC和trpE基因之间的结论，通过对携带trpC基因的各种R-prime质粒进行缺失图谱分析得到了证实。绿脓菌素R2基因簇进一步定位在两个相邻的16 kb和8.0 kb的HindIII片段上。R型绿脓菌素基因被完全缺失的PML14菌株只有一个11 kb的HindIII片段。该11 kb片段与PAO的两个HindIII片段之间的异源双链体显示，绿脓菌素R2基因簇是一个13 kb长的插入片段。
