Guesdon J L, Velarde F N, Avrameas S
Ann Immunol (Paris). 1983 Mar-Apr;134C(2):265-74. doi: 10.1016/s0769-2625(83)80097-x.
The possibility of using covalently prepared chimera antibodies in solid phase immunoassays was tested. The antibody specific for the antigen to be quantified was conjugated with a polyclonal or monoclonal antibody specific for an enzyme or for erythrocytes. Using these conjugates, labelling was done by enzyme/anti-enzyme or erythrocyte/anti-erythrocyte interactions. The chimera antibodies were used for quantitation of human IgE and for titration of human anti-Echinococcus granulosus antibody by solid-phase immunoassays, and were found to possess equal or higher amplification than conventionally prepared enzyme-antibody conjugates. A washing device for erythroadsorption test is also described. It allows such a test to be expressed in quantitative terms, as is the case in enzyme-immunoassays.
对在固相免疫测定中使用共价制备的嵌合抗体的可能性进行了测试。针对待定量抗原的特异性抗体与针对酶或红细胞的多克隆或单克隆抗体缀合。使用这些缀合物，通过酶/抗酶或红细胞/抗红细胞相互作用进行标记。嵌合抗体用于通过固相免疫测定定量人IgE和滴定人抗细粒棘球绦虫抗体，并且发现其具有与传统制备的酶-抗体缀合物相同或更高的放大倍数。还描述了一种用于红细胞吸附试验的洗涤装置。它使这种试验能够以定量方式表示，就像酶免疫测定一样。
