Bollen M, Hue L, Stalmans W
Biochem J. 1983 Mar 15;210(3):783-7. doi: 10.1042/bj2100783.
The effects of glucose on phosphorylase and glycogen synthase were investigated in hepatocytes isolated from acutely (40 h) and chronically (90 h) alloxan-diabetic rats. The glucose-induced inactivation of phosphorylase proceeded normally in all conditions. The ensuing activation of glycogen synthase was slightly blunted in acute diabetes, but became virtually absent in 72 h diabetes of similar severity. In hepatocytes from rats with various degrees of chronic diabetes, the maximal activation of glycogen synthase (at 60 mM-glucose) was inversely correlated with the plasma glucose concentration.
研究了葡萄糖对从急性(40小时)和慢性(90小时)四氧嘧啶糖尿病大鼠分离的肝细胞中磷酸化酶和糖原合酶的影响。在所有条件下,葡萄糖诱导的磷酸化酶失活均正常进行。随后糖原合酶的激活在急性糖尿病中略有减弱,但在严重程度相似的72小时糖尿病中几乎消失。在患有不同程度慢性糖尿病的大鼠的肝细胞中,糖原合酶的最大激活(在60 mM葡萄糖时)与血糖浓度呈负相关。