Carabaza A, Ciudad C J, Baqué S, Guinovart J J
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Spain.
FEBS Lett. 1992 Jan 20;296(2):211-4. doi: 10.1016/0014-5793(92)80381-p.
2-Deoxyglucose and 5-thioglucose, in the same fashion as glucose, cause the inactivation of the rat hepatocyte glycogen phosphorylase and the activation of glycogen synthase. However, 6-deoxyglucose and 1,5-anhydroglucitol inactivate phosphorylase without increasing the activation state of glycogen synthase. With 3-O-methylglucose no changes in the activity of these enzymes occurred. These results prove that while glucose is the molecule that triggers the inactivation of phosphorylase, glucose 6-phosphate is the signal for glucose synthase activation and that a metabolite control of the activation state of glycogen synthase is operative in hepatocytes.
2-脱氧葡萄糖和5-硫代葡萄糖与葡萄糖一样，可导致大鼠肝细胞糖原磷酸化酶失活并激活糖原合酶。然而，6-脱氧葡萄糖和1,5-脱水葡萄糖醇可使磷酸化酶失活，而不会增加糖原合酶的激活状态。3-O-甲基葡萄糖对这些酶的活性没有影响。这些结果证明，虽然葡萄糖是触发磷酸化酶失活的分子，但6-磷酸葡萄糖是葡萄糖合酶激活的信号，并且糖原合酶激活状态的代谢物控制在肝细胞中起作用。
