Aigueperse J, Ferezou J, Chevallier F
Biochimie. 1983 Apr-May;65(4-5):275-81. doi: 10.1016/s0300-9084(83)80279-x.
The kinetics of free and esterified cholesterol labeling were studied in the plasma lipoproteins of three groups of six adult Large White sows, after either an intravenous injection of autologous red cells previously labeled with [3H]-cholesterol, an intravenous injection of [14C]-acetate, or a [14C]-cholesterol labeled meal. The specific radioactivities became equal in plasma and red cell cholesterol about 96 hours after each pulse of radioactive cholesterol. This finding indicates that red cell cholesterol is completely exchangeable in vivo, with a turnover time of 8.5 hours. The VLDL were shown to play a preferential role in the transport in the plasma of newly synthetized cholesterol. This role is shared with chylomicrons in the transport of absorbed dietary cholesterol, which appears in the plasma mainly as esterified cholesterol. Cholesteryl esters of VLDL are not the main source for those of LDL, which could be labeled by intraplasmatic exchanges or transfers of esterified cholesterol.
在三组每组六头成年大白母猪的血浆脂蛋白中，研究了游离胆固醇和酯化胆固醇标记的动力学。实验分别采用静脉注射预先用[3H] -胆固醇标记的自体红细胞、静脉注射[14C] -乙酸盐或喂食[14C] -胆固醇标记的饲料。每次放射性胆固醇脉冲后约96小时，血浆和红细胞胆固醇中的比放射性变得相等。这一发现表明，红细胞胆固醇在体内是完全可交换的，周转时间为8.5小时。研究表明，极低密度脂蛋白（VLDL）在新合成胆固醇的血浆运输中起优先作用。在吸收的膳食胆固醇的运输中，乳糜微粒也发挥同样的作用，膳食胆固醇在血浆中主要以酯化胆固醇的形式出现。极低密度脂蛋白的胆固醇酯不是低密度脂蛋白胆固醇酯的主要来源，低密度脂蛋白的胆固醇酯可通过血浆中酯化胆固醇的交换或转移进行标记。
