Belen'kiĭ D M, Shaptseva V N
Biokhimiia. 1983 May;48(5):851-6.
The antibodies were prepared from antisera of rabbits immunized with bovine serum albumin containing covalently bound p-aminophenyl-beta-D-galactopyranoside (APG) and purified by affinity chromatography on APG-containing ovalbumin immobilized by BrCN-activated Sepharose 4B. The antibodies possessed a selective specificity for APG and interacted with different APG-containing proteins, including APG-containing lysosomal alpha-glucosidase. The purified antibodies are immunoglobulins of G type as was determined from the molecular weights of native and dissociated antibodies and from the immunochemical assays with antibodies against rabbit IgG.
这些抗体是从用含有共价结合对氨基苯基-β-D-吡喃半乳糖苷（APG）的牛血清白蛋白免疫的兔抗血清中制备的，并通过在由溴化氰活化的琼脂糖4B固定的含APG的卵清蛋白上进行亲和层析纯化。这些抗体对APG具有选择性特异性，并与不同的含APG蛋白相互作用，包括含APG的溶酶体α-葡萄糖苷酶。从天然和解离抗体的分子量以及用抗兔IgG抗体的免疫化学分析确定，纯化的抗体是G型免疫球蛋白。
