McKolanis J R, Veltri R W
Cancer Immunol Immunother. 1983;15(2):131-7. doi: 10.1007/BF00199704.
A tumor-associated membrane antigen (TAMA) has been solubilized from the C57B1/6 murine Lewis lung tumor using the detergent Triton X100. Xenoantiserum prepared in rabbits was used to identify this tumor antigen in Triton extracts by double immunodiffusion in agar. The antigen was partially purified using a combination of DEAE and Sephadex G200 chromatography followed by sucrose gradient isoelectric focusing. Isoelectric focusing also showed the antigen to have a pI at pH 4.7. Sodium dodecylsulfate polyacrylamide gel electrophoresis indicated the antigen to be of 37,000 molecular weight. The 125I protein A antibody-binding assay was used to further characterize the specificity of the antigen using rabbit antisera to the Triton extract. The same assay system also detected a specific antitumor humoral response in syngeneic mice immunized with the Triton extract.
利用去污剂曲拉通X-100从C57B1/6小鼠Lewis肺癌中溶解出一种肿瘤相关膜抗原（TAMA）。用兔制备的异种抗血清通过琼脂双向免疫扩散法在曲拉通提取物中鉴定这种肿瘤抗原。采用DEAE和葡聚糖凝胶G200柱层析相结合，随后进行蔗糖梯度等电聚焦的方法对抗原进行部分纯化。等电聚焦也显示该抗原的等电点在pH 4.7。十二烷基硫酸钠聚丙烯酰胺凝胶电泳表明该抗原分子量为37000。采用125I蛋白A抗体结合试验，用兔抗曲拉通提取物血清进一步鉴定该抗原的特异性。同一检测系统也在接种曲拉通提取物的同基因小鼠中检测到特异性抗肿瘤体液反应。
