Braatz J A, Scharfe T R, Princler G L, McIntire K R
Oncodev Biol Med. 1982;3(2-3):169-77.
A human lung tumor-associated antigen has been identified by Ouchterlony analysis in 86% of lung tumor extracts of all histologic types. It was not detected by this method in extracts of normal adult or fetal lung, 12/13 other tumors, normal tissues or in normal serum. The antigen was purified from the extract of an undifferentiated small cell lung carcinoma by DEAE-cellulose. Sephadex G-200, and antibody affinity chromatography. The purified antigen exhibited size and charge heterogeneity, yet all isolated forms produced precipitin lines of identity with each other, as well as with an extract of an unrelated squamous cell carcinoma of the lung. The major form had a Mr of 150 000 by gel filtration and 81 700 by detergent gel electrophoresis. By radioimmunoassay, elevated levels of antigen were detected in 0/15 normal sera, 7/13 stage I and 12/15 stage III lung cancer sera. The urine of a different patient with a small cell lung carcinoma contained a related antigenic activity, but the major component was 30 000-50 000 daltons. The antigen has also been detected in extracts of a continuous cell line from a large cell carcinoma of the lung (ChaGo). The antigen is being evaluated for its usefulness as a lung tumor marker.
通过免疫双扩散分析，在所有组织学类型的86%的肺肿瘤提取物中鉴定出一种人肺肿瘤相关抗原。在正常成人或胎儿肺组织提取物、13种其他肿瘤中的12种、正常组织或正常血清中，用这种方法未检测到该抗原。通过DEAE - 纤维素、葡聚糖凝胶G - 200和抗体亲和层析从未分化小细胞肺癌提取物中纯化该抗原。纯化后的抗原表现出大小和电荷的异质性，但所有分离形式彼此之间以及与一种不相关的肺鳞状细胞癌提取物都产生了相同的沉淀线。通过凝胶过滤法测得主要形式的相对分子质量为150000，通过去污剂凝胶电泳法测得为81700。通过放射免疫测定，在15份正常血清中的0份、13份I期肺癌血清中的7份和15份III期肺癌血清中的12份中检测到抗原水平升高。一名患有小细胞肺癌的不同患者的尿液中含有相关的抗原活性，但主要成分的分子量为30000 - 50000道尔顿。在一株来自肺大细胞癌的连续细胞系（ChaGo）的提取物中也检测到了该抗原。目前正在评估该抗原作为肺肿瘤标志物的实用性。
