Gillies S D, Morrison S L, Oi V T, Tonegawa S
Cell. 1983 Jul;33(3):717-28. doi: 10.1016/0092-8674(83)90014-4.
We have studied the DNA sequences required for high level expression of a cloned heavy chain immunoglobulin gene stably introduced into mouse myeloma cells by DNA transfection. We found that DNA sequences derived from the germ line JH-C mu region are required for accurate and efficient transcription from a functionally rearranged VH promoter. Similar to viral transcriptional enhancer elements, these cellular sequences stimulate transcription from either the homologous VH gene segment promoter or a heterologous SV40 promoter. They are active when placed on the 5' or 3' side of the rearranged VH gene segment and they function when their orientation is reversed. However, unlike viral enhancers, the Ig gene enhancer appears to act in a tissue-specific manner, since it is active in mouse B cells but not in mouse fibroblasts. The nucleotide sequence of the Ig enhancer region contains repeating elements that closely resemble sequence the possible role of tissue-specific transcription in cell differentiation and malignant transformation.
我们研究了通过DNA转染稳定导入小鼠骨髓瘤细胞的克隆重链免疫球蛋白基因高水平表达所需的DNA序列。我们发现，来自种系JH-Cμ区域的DNA序列对于从功能重排的VH启动子进行准确而有效的转录是必需的。与病毒转录增强子元件相似，这些细胞序列可刺激来自同源VH基因片段启动子或异源SV40启动子的转录。当置于重排的VH基因片段的5'或3'侧时它们具有活性，并且当它们的方向颠倒时它们也能发挥作用。然而，与病毒增强子不同，Ig基因增强子似乎以组织特异性方式起作用，因为它在小鼠B细胞中具有活性而在小鼠成纤维细胞中无活性。Ig增强子区域的核苷酸序列包含与序列紧密相似的重复元件，这些序列可能在细胞分化和恶性转化中起组织特异性转录的作用。
