Friedman B M, Yasbin R E
Mol Gen Genet. 1983;190(3):481-6. doi: 10.1007/BF00331080.
An isogenic set of DNA repair-proficient and -deficient strains of B. subtilis, cured of all prophages, were constructed and analyzed for their sensitivities to selected mutagens. The results demonstrated that the lethal damage caused by ultraviolet (UV) radiation and by 4-nitroquinoline-1-oxide (4NQO) were repaired by the bacterial excision and/or recombination repair systems. In contrast, the lethal damages caused by ethyl methane sulfonate (EMS) and methyl methane sulfonate (MMS) were removed from the DNA by the recombination repair system of the bacteria, and not by the excision repair system. Significantly, the bacteria required both a functional recombination repair system and a functional excision repair system in order to remove the DNA damage caused by the bifunctional alkylating agent mitomycin C (MC).
构建了一组经噬菌体清除的枯草芽孢杆菌DNA修复功能正常和缺陷的同基因菌株，并分析了它们对选定诱变剂的敏感性。结果表明，紫外线（UV）辐射和4-硝基喹啉-1-氧化物（4NQO）造成的致死性损伤可通过细菌切除和/或重组修复系统修复。相比之下，甲磺酸乙酯（EMS）和甲磺酸甲酯（MMS）造成的致死性损伤可通过细菌的重组修复系统从DNA中去除，而不是通过切除修复系统。值得注意的是，细菌需要功能性重组修复系统和功能性切除修复系统才能去除双功能烷化剂丝裂霉素C（MC）造成的DNA损伤。
