一种具有抗肺炎克雷伯菌多糖K30和K33中3,4-丙酮酸化半乳糖抗体活性的人单克隆IgM（蛋白WEA）Fv区的氨基酸序列。
Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.
作者信息
Goñi F, Frangione B
出版信息
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4837-41. doi: 10.1073/pnas.80.15.4837.
Abstract
We have determined the amino acid sequence of the Fv [variable heavy (VH) and variable light (VL)] region of a human monoclonal IgM-kappa with antibody activity against 3,4-pyruvylated galactose, isolated from the plasma of patient WEA with Waldenström macroglobulinemia. The VH region has 114 residues, belongs to subgroup III, and has a very short third complementarity-determining region (CDR3), probably due to a small D segment/or an unusual D-J rearrangement (D, diversity; J, joining). The VL region has 108 residues and belongs to subgroup V kappa I. Compared to other members of the human VHIII and V kappa I families, WEA Fv does not appear to have significant differences within the framework residues but has unique CDRs that might be responsible for the particular antibody activity. Another IgM-kappa (GAL), which has an as-yet-undetermined antibody activity, shares a striking homology in V kappa with WEA, including an identical CDR1.
摘要
我们已确定从患有华氏巨球蛋白血症的患者WEA血浆中分离出的、具有抗3,4-丙酮酸化半乳糖抗体活性的人单克隆IgM-κ的Fv[重链可变区（VH）和轻链可变区（VL）]区域的氨基酸序列。VH区域有114个残基，属于III亚组，且具有非常短的第三互补决定区（CDR3），这可能是由于D片段较小/或存在不寻常的D-J重排（D，多样性；J，连接）。VL区域有108个残基，属于VκI亚组。与人类VHIII和VκI家族的其他成员相比，WEA Fv在框架残基内似乎没有显著差异，但具有独特的互补决定区，这可能是其特定抗体活性的原因。另一种IgM-κ（GAL），其抗体活性尚未确定，在Vκ区域与WEA具有显著的同源性，包括相同的CDR1。
相似文献
1
Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.一种具有抗肺炎克雷伯菌多糖K30和K33中3,4-丙酮酸化半乳糖抗体活性的人单克隆IgM（蛋白WEA）Fv区的氨基酸序列。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4837-41. doi: 10.1073/pnas.80.15.4837.
2
Effect of pH on the precipitin reaction of human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides containing 3,4-pyruvylated D-galactose and 4,6-pyruvylated D-galactose.
Mol Immunol. 1982 Apr;19(4):609-18. doi: 10.1016/0161-5890(82)90230-9.
3
Amino acid sequence of the variable region of a human mu chain: location of a possible JH segment.人μ链可变区的氨基酸序列：一个可能的JH片段的位置。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3239-43. doi: 10.1073/pnas.77.6.3239.
4
Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose.对含有3,4-丙酮酰化-D-半乳糖和4,6-丙酮酰化-D-半乳糖的克雷伯菌K多糖具有特异性的人单克隆巨球蛋白。
J Exp Med. 1980 Oct 1;152(4):979-95. doi: 10.1084/jem.152.4.979.
5
Expression of monovalent fragments derived from a human IgM autoantibody in E. coli. The input of the somatically mutated CDR1/CDR2 and of the CDR3 into antigen binding specificity.源自人IgM自身抗体的单价片段在大肠杆菌中的表达。体细胞突变的互补决定区1/互补决定区2以及互补决定区3对抗原结合特异性的贡献。
Immunobiology. 1995 Aug;193(5):400-19. doi: 10.1016/S0171-2985(11)80427-4.
6
Cloning of cDNAs encoding the variable domains of antibody BrE-3 and construction of a chimeric antibody.编码抗体BrE-3可变区的cDNA克隆及嵌合抗体的构建。
Hybridoma. 1993 Feb;12(1):15-23. doi: 10.1089/hyb.1993.12.15.
7
Variable region genes of human monoclonal autoantibodies to histones H2A and H2B from a systemic lupus erythematosus patient.一名系统性红斑狼疮患者针对组蛋白H2A和H2B的人单克隆自身抗体的可变区基因
Mol Immunol. 2005 Feb;42(3):311-7. doi: 10.1016/j.molimm.2004.07.008.
8
Amino acid sequence of a phosphocholine-binding antibody from an immune defective CBA/N mouse employing the T15 VH region associated with unusual DH, JH, and V kappa segments.利用与异常重链多样性基因（DH）、重链连接基因（JH）和κ轻链可变区（Vκ）片段相关的T15重链可变区（VH），从免疫缺陷的CBA/N小鼠中获得的一种磷酸胆碱结合抗体的氨基酸序列。
J Immunol. 1984 Mar;132(3):1544-9.
9
Clonal analysis of a human antibody response. III. Nucleotide sequences of monoclonal IgM, IgG, and IgA to rabies virus reveal restricted V kappa gene utilization, junctional V kappa J kappa and V lambda J lambda diversity, and somatic hypermutation.人类抗体应答的克隆分析。III. 针对狂犬病病毒的单克隆IgM、IgG和IgA的核苷酸序列揭示了受限的Vκ基因利用、连接区VκJκ和VλJλ多样性以及体细胞超突变。
J Immunol. 1998 Sep 15;161(6):2895-905.
10
Characteristics of murine monoclonal anti-CD4. Epitope recognition, idiotype expression, and variable region gene sequence.小鼠单克隆抗CD4的特性。表位识别、独特型表达及可变区基因序列。
J Immunol. 1992 Nov 15;149(10):3247-53.
引用本文的文献
1
Continuing pursuit for ideal systemic anticancer radiotherapeutics.持续追求理想的全身抗肿瘤放射疗法。
Invest New Drugs. 2012 Oct;30(5):2050-65. doi: 10.1007/s10637-011-9758-6. Epub 2011 Oct 18.
2
Sequence similarities among kappa IIIb chains of monoclonal human IgM kappa autoantibodies.单克隆人IgMκ自身抗体的κIIIb链之间的序列相似性。
J Exp Med. 1984 Sep 1;160(3):893-904. doi: 10.1084/jem.160.3.893.
3
Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype.含有显性16/6独特型的人单克隆狼疮自身抗体重链和轻链的NH2末端氨基酸序列的同源性。
J Clin Invest. 1985 Apr;75(4):1138-43. doi: 10.1172/JCI111808.
4
The detection of a common idiotype of anti-DNA antibodies in the sera of patients with monoclonal gammopathies.在单克隆丙种球蛋白病患者血清中检测抗DNA抗体的一种常见独特型。
J Clin Immunol. 1986 May;6(3):194-204. doi: 10.1007/BF00918699.
5
Idiotypic and subgroup analysis of human monoclonal rheumatoid factors. Implications for structural and genetic basis of autoantibodies in humans.人单克隆类风湿因子的独特型及亚组分析。对人类自身抗体结构和遗传基础的启示。
J Clin Invest. 1988 Aug;82(2):469-75. doi: 10.1172/JCI113620.
6
New roles for rheumatoid factor.类风湿因子的新作用。
J Clin Invest. 1991 Feb;87(2):379-83. doi: 10.1172/JCI115007.
7
Biosynthetic antibody binding sites: development of a single-chain Fv model based on antidinitrophenol IgA myeloma MOPC 315.生物合成抗体结合位点：基于抗二硝基苯酚 IgA 骨髓瘤 MOPC 315 的单链 Fv 模型的开发
J Protein Chem. 1991 Dec;10(6):669-83. doi: 10.1007/BF01025718.
本文引用的文献
1
Metabolism of nicotinic acid and related compounds in man and rat.人体和大鼠中烟酸及相关化合物的代谢
Biochem J. 1953 Jan;53(2):286-94. doi: 10.1042/bj0530286.
2
Human immunoglobulin D segments encoded in tandem multigenic families.串联多基因家族中编码的人类免疫球蛋白D区段。
Nature. 1981 Dec 17;294(5842):631-5. doi: 10.1038/294631a0.
3
Gene conversion: some implications for immunoglobulin genes.基因转换：对免疫球蛋白基因的一些影响。
Cell. 1981 Jun;24(3):592-4. doi: 10.1016/0092-8674(81)90082-9.
4
A cDNA sequence encoding a rabbit heavy chain variable region of the VHa2 allotype showing homologies with human heavy chain sequences.一个编码兔VHa2同种异型重链可变区的cDNA序列，该序列与人重链序列具有同源性。
Nature. 1982 Nov 4;300(5887):74-6. doi: 10.1038/300074a0.
5
Fourteen nucleotides in the second complementarity-determining region of a human heavy-chain variable region gene are identical with a sequence in a human D minigene.人类重链可变区基因的第二个互补决定区中的14个核苷酸与人类D小基因中的一个序列相同。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5031-2. doi: 10.1073/pnas.79.16.5031.
6
Amino acid sequence of the variable regions of light chains from two idiotypically cross-reactive human IgM anti-gamma-globulins of the Wa group.来自Wa组两种独特型交叉反应性人IgM抗γ球蛋白轻链可变区的氨基酸序列。
Biochemistry. 1981 Sep 29;20(20):5816-22. doi: 10.1021/bi00523a026.
7
Immunoglobulin genes undergo multiple sequence rearrangements during differentiation.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:867-78. doi: 10.1101/sqb.1981.045.01.104.
8
Somatic reorganization of immunoglobulin genes during lymphocyte differentiation.淋巴细胞分化过程中免疫球蛋白基因的体细胞重排。
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:839-58. doi: 10.1101/sqb.1981.045.01.102.
9
Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.免疫球蛋白重链连接区基因序列及其在抗体多样性产生中的作用。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):509-13. doi: 10.1073/pnas.78.1.509.
10
Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.免疫球蛋白重链基因多样性DNA片段（D）的鉴定及核苷酸序列
Nature. 1981 Apr 16;290(5807):562-5. doi: 10.1038/290562a0.
Zotero 插件