Custer E M, Myers J L, Poffenbarger P L, Schoen I
Am J Clin Pathol. 1983 Sep;80(3):375-80. doi: 10.1093/ajcp/80.3.375.
The storage stability of 3-hydroxybutyrate in whole blood, serum, and plasma was evaluated. The levels of 3-hydroxybutyrate were measured using an enzymatic rate reaction of the specific 3-hydroxybutyrate dehydrogenase with the NAD-NADH coupled reaction. 3-Hydroxybutyrate was found to be a stable analyte in whole blood, plasma, and serum. The long-term stability of beta-hydroxybutyrate allows ample time for separation of blood components and offers storage of samples to meet quality control needs as well as the possibility of mailing specimens. Further studies indicate that NaF plasma, heparin plasma, and serum are the preferred specimens, because both EDTA and oxalate showed the most significant interference with the determination of 3-hydroxybutyrate.
评估了全血、血清和血浆中3-羟基丁酸酯的储存稳定性。采用特定的3-羟基丁酸脱氢酶与NAD-NADH偶联反应的酶促速率反应来测定3-羟基丁酸酯的水平。结果发现，3-羟基丁酸酯在全血、血浆和血清中是一种稳定的分析物。β-羟基丁酸酯的长期稳定性为血液成分的分离留出了充足的时间，并为满足质量控制需求储存样本以及邮寄标本提供了可能性。进一步的研究表明，氟化钠血浆、肝素血浆和血清是首选标本，因为乙二胺四乙酸（EDTA）和草酸盐对3-羟基丁酸酯的测定显示出最显著的干扰。
