尿苷三磷酸（UTP）对金色链霉菌肽链起始的刺激作用。

Stimulatory effect of UTP on peptide chain initiation in Streptomyces aureofaciens.

作者信息

Mikulík K, Jiránová A, Weiser J

出版信息

Biochem Biophys Res Commun. 1983 Jul 18;114(1):222-9. doi: 10.1016/0006-291x(83)91616-9.

Abstract

The formation of the 30S and 70S initiation complex in Streptomyces aureofaciens differs from that in E.coli and B.stearothermophilus with respect to the requirement for nucleotide triphosphates for the maximum activity. In the presence of GTP and initiation factors from S.aureofaciens the codon specific binding of fMet-tRNA to ribosomes of S.aureofaciens was stimulated by ATP or UTP. UTP exhibited the most significant effect increasing the binding about 3.5 times, whereas CTP had no effect on the reaction. The stimulatory effect of UTP is GTP-dependent and was not observed in experiments with E.coli ribosomes.

摘要

金色链霉菌中30S和70S起始复合物的形成在最大活性所需的三磷酸核苷酸方面与大肠杆菌和嗜热栖热菌不同。在存在GTP和金色链霉菌的起始因子的情况下，甲硫氨酸- tRNA与金色链霉菌核糖体的密码子特异性结合受到ATP或UTP的刺激。UTP表现出最显著的效果，使结合增加约3.5倍，而CTP对该反应没有影响。UTP的刺激作用依赖于GTP，并且在大肠杆菌核糖体的实验中未观察到。