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[体外培养的小鼠乳腺癌细胞中Thy 1胸腺细胞分化抗原的表达]

[Expression of Thy 1 thymocyte differentiation antigen in mouse mammary cancer cells in vitro].

作者信息

Kriukova I N, Litvinov S V

出版信息

Biull Eksp Biol Med. 1983 Aug;96(8):81-4.

PMID:6411148
Abstract

Allogeneic AKR-anti C3H Thy 1.2 antigen serum and monoclonal anti-Thy 1.2 and anti-Thy 1.1 antigen antibodies were used to study the expression of lymphocyte differentiation antigen in a clonal mammary carcinoma cell line originated from a GR/mt stable cell line. Both allogeneic antiserum and monoclonal anti-Thy 1.2 (but not Thy 1.1) antibody were active with the hormone-treated fixed cells in an indirect immunofluorescence test. However, antigen on the cellular membranes could be detected only with the use of allogeneic (but not with monoclonal antibody) anti-Thy 1.2 serum.

摘要

使用同种异体AKR抗C3H Thy 1.2抗原血清以及单克隆抗Thy 1.2和抗Thy 1.1抗原抗体,来研究源自GR/mt稳定细胞系的克隆性乳腺癌细胞系中淋巴细胞分化抗原的表达。在间接免疫荧光试验中,同种异体抗血清和单克隆抗Thy 1.2(而非Thy 1.1)抗体对经激素处理的固定细胞均有活性。然而,仅使用同种异体(而非单克隆抗体)抗Thy 1.2血清才能检测到细胞膜上的抗原。

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