犬弓首蛔虫幼虫排泄分泌抗原的纯化与鉴定

Immunology. 1983 Sep;50(1):113-20.

Excretory/secretory (ES) antigen of Toxocara canis larvae was isolated from the culture medium of second-stage larvae by gel filtration. The antigen induced production of not only IgM or IgG but also IgE antibody. The antigen, when treated with guanidine hydrochloride and 2-mercaptoethanol, lost its allergenic activity but retained an ability to induce IgM or IgG antibody. Purified antigen was of glycoprotein nature and had a molecular weight of 35,000. The whole antigen showed a cross-reaction with the serum from Ascaris suum-infected mice. The antigen did not contain phosphorylcholine as a structural component.

犬弓首蛔虫幼虫的排泄/分泌（ES）抗原通过凝胶过滤从二期幼虫的培养基中分离得到。该抗原不仅能诱导IgM或IgG产生，还能诱导IgE抗体产生。用盐酸胍和2-巯基乙醇处理后，该抗原失去了过敏活性，但仍保留诱导IgM或IgG抗体的能力。纯化后的抗原具有糖蛋白性质，分子量为35000。整个抗原与感染猪蛔虫小鼠的血清有交叉反应。该抗原不含磷酸胆碱作为结构成分。