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犬弓首蛔虫幼虫排泄分泌抗原的纯化与鉴定

Purification and characterization of excretory and secretory antigen of Toxocara canis larvae.

作者信息

Sugane K, Oshima T

出版信息

Immunology. 1983 Sep;50(1):113-20.

PMID:6411609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1454224/
Abstract

Excretory/secretory (ES) antigen of Toxocara canis larvae was isolated from the culture medium of second-stage larvae by gel filtration. The antigen induced production of not only IgM or IgG but also IgE antibody. The antigen, when treated with guanidine hydrochloride and 2-mercaptoethanol, lost its allergenic activity but retained an ability to induce IgM or IgG antibody. Purified antigen was of glycoprotein nature and had a molecular weight of 35,000. The whole antigen showed a cross-reaction with the serum from Ascaris suum-infected mice. The antigen did not contain phosphorylcholine as a structural component.

摘要

犬弓首蛔虫幼虫的排泄/分泌（ES）抗原通过凝胶过滤从二期幼虫的培养基中分离得到。该抗原不仅能诱导IgM或IgG产生，还能诱导IgE抗体产生。用盐酸胍和2-巯基乙醇处理后，该抗原失去了过敏活性，但仍保留诱导IgM或IgG抗体的能力。纯化后的抗原具有糖蛋白性质，分子量为35000。整个抗原与感染猪蛔虫小鼠的血清有交叉反应。该抗原不含磷酸胆碱作为结构成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/1a63573404ae/immunology00210-0119-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/e6272a859dca/immunology00210-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/c2c8f9fde74f/immunology00210-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/1a63573404ae/immunology00210-0119-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/e6272a859dca/immunology00210-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/c2c8f9fde74f/immunology00210-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f30b/1454224/1a63573404ae/immunology00210-0119-c.jpg

相似文献

1
Purification and characterization of excretory and secretory antigen of Toxocara canis larvae.犬弓首蛔虫幼虫排泄分泌抗原的纯化与鉴定
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2
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In vitro production of Toxocara canis excretory-secretory (TES) antigen.犬弓首蛔虫排泄分泌（TES）抗原的体外生产
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The Carbohydrate-linked Phosphorylcholine of the Parasitic Nematode Product ES-62 Modulates Complement Activation.寄生线虫产物ES-62的糖基化磷酸胆碱可调节补体激活。

本文引用的文献

1
Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent.用德雷伍德蒽酮试剂定量测定碳水化合物
Science. 1948 Mar 5;107(2775):254-5. doi: 10.1126/science.107.2775.254.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
IMMUNOCHEMICAL STUDIES ON ANTIGEN PREPARATIONS FROM STAPHYLOCOCCUS AUREUS. 1. ISOLATION AND CHEMICAL CHARACTERIZATION OF ANTIGEN A.金黄色葡萄球菌抗原制剂的免疫化学研究。1. 抗原A的分离与化学特性分析
J Biol Chem. 2016 May 27;291(22):11939-53. doi: 10.1074/jbc.M115.702746. Epub 2016 Apr 4.
4
In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products.马副蛔虫幼虫的体外培养及寄生虫排泄分泌产物的初步研究
Parasitol Res. 2014 Nov;113(11):4217-24. doi: 10.1007/s00436-014-4097-0. Epub 2014 Sep 11.
5
Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans.犬弓首蛔虫种特异性排泄分泌抗原（TcES-57）的鉴定及用于诊断内脏幼虫移行症的双夹心ELISA的开发。
Korean J Parasitol. 2007 Mar;45(1):19-26. doi: 10.3347/kjp.2007.45.1.19.
6
Immune reactions and allergy in experimental anisakiasis.实验性异尖线虫病中的免疫反应与过敏
Korean J Parasitol. 2006 Dec;44(4):271-83. doi: 10.3347/kjp.2006.44.4.271.
7
Highlights of human toxocariasis.人类弓蛔虫病要点
Korean J Parasitol. 2001 Mar;39(1):1-11. doi: 10.3347/kjp.2001.39.1.1.
8
Effects of monoclonal antibodies to adhesion molecules on eosinophilic myocarditis in Toxocara canis-infected CBA/J mice.抗粘附分子单克隆抗体对犬弓首蛔虫感染的CBA/J小鼠嗜酸性粒细胞性心肌炎的影响。
Clin Exp Immunol. 1998 Nov;114(2):236-44. doi: 10.1046/j.1365-2249.1998.00661.x.
9
Stage-specific secreted antigens of the parasitic larval stages of the nematode Ascaris.线虫蛔虫寄生幼虫阶段的阶段特异性分泌抗原。
Immunology. 1986 Jul;58(3):515-22.
10
Application of the western blotting procedure for the immunodiagnosis of human toxocariasis.蛋白质印迹法在人体弓蛔虫病免疫诊断中的应用。
Parasitol Res. 1991;77(8):697-702. doi: 10.1007/BF00928685.
Acta Pathol Microbiol Scand. 1964;61:588-96. doi: 10.1111/apm.1964.61.4.588.
4
Organ disturbution of Toxocara canis larvae in normal mice and in mice previously infected with Toxocara, Ascaris or Trichinella.犬弓首蛔虫幼虫在正常小鼠以及先前感染过犬弓首蛔虫、蛔虫或旋毛虫的小鼠体内的器官分布。
Tex Rep Biol Med. 1962;20:651-7.
5
Standardization of techniques for infecting mice with Toxocara canis and observations on the normal migration routes of the larvae.犬弓首蛔虫感染小鼠技术的标准化及幼虫正常移行途径的观察
J Parasitol. 1961 Aug;47:652-6.
6
The latex-fixation test. III. Agglutination test for C-reactive protein and comparison with the capillary precipitin method.胶乳凝集试验。III. C反应蛋白的凝集试验及其与毛细管沉淀素法的比较。
Am J Clin Pathol. 1957 Dec;28(6):611-7. doi: 10.1093/ajcp/28.6.611.
7
Recovery of large numbers of eosinophils from mice infected with Toxocara canis.从感染犬弓首蛔虫的小鼠体内回收大量嗜酸性粒细胞。
Am J Trop Med Hyg. 1980 Sep;29(5):799-802. doi: 10.4269/ajtmh.1980.29.799.
8
Preparation of a highly purified allergen from Dirofilaria immitis. Reaginic antibody formation in mice.从犬恶丝虫制备高纯度变应原。小鼠体内反应素抗体的形成。
Immunology. 1981 Mar;42(3):363-70.
9
Hemagglutination test for the detection of antibodies specific for Ascaris and Toxocara antigens in patients with suspected visceral larva migrans.用于检测疑似内脏幼虫移行症患者中针对蛔虫和弓首蛔虫抗原的特异性抗体的血凝试验。
Am J Trop Med Hyg. 1974 May;23(3):378-84. doi: 10.4269/ajtmh.1974.23.378.
10
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.平板凝胶上噬菌体T7早期RNA和蛋白质的分析。
J Mol Biol. 1973 Sep 15;79(2):237-48. doi: 10.1016/0022-2836(73)90003-x.