Nierzwicki-Bauer S A, Balkwill D L, Stevens S E
J Cell Biol. 1983 Sep;97(3):713-22. doi: 10.1083/jcb.97.3.713.
The first complete three-dimensional ultrastructural reconstruction of a cyanobacterium was accomplished with high-voltage electron microscopy and computer-aided assembly of serial sections. The precise arrangement of subcellular features within the cell body was very consistent from one cell to another. Specialized inclusion bodies always occupied specific intracellular locations. The photosynthetic thylakoid membranes entirely surrounded the central portion of the cytoplasm, thereby compartmentalizing it from the rest of the cell. The thylakoid membranes formed an interconnecting network of concentric shells, merging only at the inner surface of the cytoplasmic membrane. The thylakoids were in contact with the cytoplasmic membrane at several locations, apparently to maintain the overall configuration of the thylakoid system. These results clarified several unresolved issues regarding structure-function relationships in cyanobacteria.
利用高压电子显微镜和连续切片的计算机辅助组装技术，首次完成了蓝细菌的完整三维超微结构重建。细胞体内亚细胞特征的精确排列在一个细胞与另一个细胞之间非常一致。特殊的包涵体总是占据特定的细胞内位置。光合类囊体膜完全包围了细胞质的中心部分，从而将其与细胞的其余部分分隔开来。类囊体膜形成了一个相互连接的同心壳网络，仅在细胞质膜的内表面合并。类囊体在几个位置与细胞质膜接触，显然是为了维持类囊体系统的整体构型。这些结果澄清了关于蓝细菌结构-功能关系的几个未解决问题。
