Ghiselli G, Schaefer E J, Light J A, Brewer H B
J Lipid Res. 1983 Jun;24(6):731-6.
The effect of fat feeding (100 g of cream) on the apoA-I isoproteins distribution has been analyzed by two-dimensional gel electrophoresis in the chylomicrons, VLDL, LDL, and HDL isolated from the thoracic duct lymph of patients undergoing lymph drainage for immunosuppression, Isoforms apoA-I3 and apoA-I4 are the most abundant apoA-I isoproteins in plasma lipoproteins as well as in lymph lipoproteins collected in the fasting state. Fat feeding, on the other hand, results in a marked change in the apoA-I isoform pattern in lymph chylomicrons and VLDL, with a significant increase in the relative concentration of the apoA-I1 isoform. As a result the total concentration of this isoprotein in the lymph increased. The data indicate that fat feeding is associated with major changes in the distribution of the apoA-I isoforms in the lymph (d less than 1.006 g/ml lipoproteins), which may be of significance in their plasma catabolism.
通过二维凝胶电泳分析了脂肪喂养（100克奶油）对从接受免疫抑制淋巴引流患者的胸导管淋巴中分离出的乳糜微粒、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）中载脂蛋白A-I同工蛋白分布的影响。载脂蛋白A-I3和载脂蛋白A-I4同工型是血浆脂蛋白以及空腹状态下收集的淋巴脂蛋白中最丰富的载脂蛋白A-I同工蛋白。另一方面，脂肪喂养导致淋巴乳糜微粒和VLDL中载脂蛋白A-I同工型模式发生显著变化，载脂蛋白A-I1同工型的相对浓度显著增加。结果，这种同工蛋白在淋巴中的总浓度增加。数据表明，脂肪喂养与淋巴中（密度小于1.006克/毫升脂蛋白）载脂蛋白A-I同工型分布的主要变化有关，这可能对其血浆分解代谢具有重要意义。
