Tapia-Arancibia L, Astier H
Neuroendocrinology. 1983 Aug;37(2):166-8. doi: 10.1159/000123537.
The effect of morphine and leucine enkephalin on basal and K+-induced TRH release by superfused mediobasal hypothalami in rats was investigated in an oxygenated modified Locke medium at 37 degrees C during 10 min. Both opiates (morphine, 10(-6) M; leucine enkephalin, 10(-6) M) did not modify the spontaneous release of TRH, but significantly decreased the depolarization-induced TRH release. Naloxone (10(-6) M) had no effect when added alone to the medium, but reversed the opiate inhibition of TRH release. The data suggest that endogenous opiates exert a modulatory action on TRH nerve endings in the mediobasal hypothalami, probably through presynaptic specific opiate receptors.
在37℃的含氧改良洛克培养基中，于10分钟内研究了吗啡和亮氨酸脑啡肽对大鼠离体下丘脑基底部基础状态及钾离子诱导的促甲状腺激素释放激素（TRH）释放的影响。两种阿片类药物（吗啡，10⁻⁶ M；亮氨酸脑啡肽，10⁻⁶ M）均未改变TRH的自发释放，但显著降低了去极化诱导的TRH释放。纳洛酮（10⁻⁶ M）单独加入培养基时无作用，但可逆转阿片类药物对TRH释放的抑制作用。数据表明，内源性阿片类物质可能通过突触前特异性阿片受体，对下丘脑基底部的TRH神经末梢发挥调节作用。
