两栖动物大脑中前脑啡肽原样前体的证据。
Evidence for a proenkephalin-like precursor in amphibian brain.
作者信息
Kilpatrick D L, Howells R D, Lahm H W, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5772-5. doi: 10.1073/pnas.80.18.5772.
Abstract
The mammalian proenkephalin-derived peptides [Leu]enkephalin, [Met]enkephalin, and [Met]enkephalin-Arg6-Phe7 were identified in acid extracts of the brain of Bufo marinus by using reversed-phase HPLC and specific radioimmunoassays. [Met]Enkephalin was the predominant opioid peptide present (270 pmol/g). In contrast, the octapeptide [Met]enkephalin-Arg6-Gly7-Leu8, which is also derived from mammalian proenkephalin, was not detected. The ratio of free [Met]enkephalin to [Met]enkephalin-Arg6-Phe7 was found to be 3.5 to 1, which is similar to that observed in mammalian proenkephalin-containing tissues. Together these data (i) indicate that amphibian brain contains a proenkephalin related to the mammalian precursor and (ii) establish the existence of enkephalins and proenkephalin-derived enkephalin-containing peptides in a submammalian species.
摘要
通过使用反相高效液相色谱法和特异性放射免疫分析法，在海蟾蜍大脑的酸提取物中鉴定出了源自哺乳动物前脑啡肽的肽类物质，即亮氨酸脑啡肽、甲硫氨酸脑啡肽和甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7。甲硫氨酸脑啡肽是主要存在的阿片样肽（270 pmol/g）。相比之下，同样源自哺乳动物前脑啡肽的八肽甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8未被检测到。游离甲硫氨酸脑啡肽与甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7的比例为3.5比1，这与在含有哺乳动物前脑啡肽的组织中观察到的比例相似。这些数据共同表明：（i）两栖动物大脑含有一种与哺乳动物前体相关的前脑啡肽；（ii）证实了在非哺乳动物物种中存在脑啡肽以及源自前脑啡肽的含脑啡肽肽类物质。
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本文引用的文献
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Identification of the octapeptide [Met]enkephalin -Arg6-Gly7-Leu8 in extracts of bovine adrenal medulla.牛肾上腺髓质提取物中八肽[甲硫氨酸]脑啡肽-精氨酸6-甘氨酸7-亮氨酸8的鉴定。
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Existence of Met-enkephalin-Arg6-Gly7-Leu8 with Met-enkephalin, Leu-enkephalin and Met-enkephalin-Arg6-Phe7 in the brain of guinea pig, rat and golden hamster.在豚鼠、大鼠和金黄仓鼠的大脑中，甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8与甲硫氨酸脑啡肽、亮氨酸脑啡肽和甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7共存。
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