两种肾上腺阿片样多肽：脑啡肽原加工过程中的假定中间体。
Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin.
作者信息
Stern A S, Jones B N, Shively J E, Stein S, Undenfriend S
出版信息
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1962-6. doi: 10.1073/pnas.78.3.1962.
Abstract
Two enkephalin-containing polypeptides of 3600 and 4900 daltons have been isolated from extracts of bovine adrenal medulla, purified to homogeneity, and analyzed by a combination of automated Edman degradation and enzymatic time course hydrolysis. The 4900-dalton polypeptide contains two copies of enkephalin, one an internal [Met]enkephalin sequence, the other a [Leu]enkephalin sequence at the carboxyl terminus. Sequence analysis of the 3600-dalton polypeptide has not been completed, but the polypeptide has been shown to contain a single [Met]enkephalin sequence followed by an -Arg-Phe linkage that forms the carboxyl terminus of the molecule. On the basis of these and other findings, we propose that the above enkephalin-containing polypeptides are intermediates in the biosynthesis of the enkephalins and that they are generated by posttranslational processing from a large multivalent enkephalin precursor molecule, proenkephalin. The term "multivalent" is used to indicate a polypeptide with many identical functional sequences.
摘要
从牛肾上腺髓质提取物中分离出了两种分子量分别为3600和4900道尔顿的含脑啡肽多肽，将其纯化至同质，并通过自动Edman降解和酶促时间进程水解相结合的方法进行分析。4900道尔顿的多肽包含两个脑啡肽拷贝，一个是内部的[甲硫氨酸]脑啡肽序列，另一个是位于羧基末端的[亮氨酸]脑啡肽序列。3600道尔顿多肽的序列分析尚未完成，但已证明该多肽含有一个单一的[甲硫氨酸]脑啡肽序列，其后是一个形成分子羧基末端的-精氨酸-苯丙氨酸连接。基于这些及其他发现，我们提出上述含脑啡肽多肽是脑啡肽生物合成的中间体，它们是由一个大型多价脑啡肽前体分子——前脑啡肽经翻译后加工产生的。术语“多价”用于表示具有许多相同功能序列的多肽。
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Proc Natl Acad Sci U S A. 1981 Mar;78(3):1962-6. doi: 10.1073/pnas.78.3.1962.
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A new endogenous opioid peptide from bovine adrenal medulla: isolation and amino acid sequence of a dodecapeptide (BAM-12P).一种来自牛肾上腺髓质的新型内源性阿片肽：一种十二肽（BAM - 12P）的分离及氨基酸序列
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J Neurosci. 1981 Jan;1(1):80-2. doi: 10.1523/JNEUROSCI.01-01-00080.1981.
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10
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