Wooley R E, Jones M S
Vet Microbiol. 1983 Jun;8(3):271-80. doi: 10.1016/0378-1135(83)90079-2.
The concentration of EDTA-Tris (3.22 mM EDTA and 0.05 M Tris) used as a lavage to treat otitis externa, cystitis or other persistent infections in dogs and cats, was found to prevent the growth of Pseudomonas aeruginosa, Staphylococcus aureus and beta streptococci when present in growth media. Pseudomonas aeruginosa was rapidly lysed in this solution. Escherichia coli and Proteus vulgaris grew in the presence of EDTA-Tris, but to a lesser extent than the controls without these compounds. Minimal inhibitory concentrations (MIC) for 8 antimicrobial agents with and without EDTA-Tris were determined for E. coli and P. vulgaris. A potentiation effect on E. coli (greater than 50% decrease MIC) was observed when EDTA-Tris was combined with penicillin, oxytetracycline or chloramphenicol. A similar effect was observed with P. vulgaris when combinations of EDTA-Tris plus gentamicin, oxytetracycline, polymyxin-B or triple sulfa were used. The results of this study indicate that EDTA-Tris appears to have merit in selected cases of otitis externa, cystitis or other persistent infections where lavage might be used.
用作灌洗以治疗犬猫外耳炎、膀胱炎或其他持续性感染的乙二胺四乙酸 - 三羟甲基氨基甲烷（3.22 mM乙二胺四乙酸和0.05 M三羟甲基氨基甲烷）浓度，被发现当存在于生长培养基中时可防止铜绿假单胞菌、金黄色葡萄球菌和β - 链球菌生长。铜绿假单胞菌在此溶液中迅速裂解。大肠杆菌和普通变形杆菌在乙二胺四乙酸 - 三羟甲基氨基甲烷存在下生长，但生长程度低于不含这些化合物的对照组。测定了有和没有乙二胺四乙酸 - 三羟甲基氨基甲烷时8种抗菌剂对大肠杆菌和普通变形杆菌的最小抑菌浓度（MIC）。当乙二胺四乙酸 - 三羟甲基氨基甲烷与青霉素、土霉素或氯霉素联合使用时，观察到对大肠杆菌有增效作用（MIC降低大于50%）。当使用乙二胺四乙酸 - 三羟甲基氨基甲烷加庆大霉素、土霉素、多粘菌素B或三联磺胺的组合时，普通变形杆菌也观察到类似效果。本研究结果表明，在可能使用灌洗的外耳炎、膀胱炎或其他持续性感染的特定病例中，乙二胺四乙酸 - 三羟甲基氨基甲烷似乎有价值。
