Luther Anne-Marie, Nguyen Thu Quynh, Verspohl Jutta, Waberski Dagmar
Unit for Reproductive Medicine, Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany.
Institute for Microbiology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
Antibiotics (Basel). 2021 Oct 29;10(11):1319. doi: 10.3390/antibiotics10111319.
Antibiotic use in semen extenders for livestock may contribute to the development and spreading of multi-drug resistance. Antimicrobial control in semen doses for artificial insemination of pigs is indispensable due to the relatively high storage temperature (17 °C). The objectives of this study were first, to examine whether the antimicrobial capacity differs between antibiotic-free extenders and second, to determine whether an antimicrobial active extender provides the possibility to reduce antibiotics. Antibiotic-free semen extenders Beltsville Thawing Solution (BTS) and Androstar Premium were inoculated at 10 to 10 CFU/mL with four pure bacterial strains isolated from boar ejaculates or a mixture thereof, and then stored for 144 h at 17 °C. Bacterial counts after aerobic culture decreased in BTS up to one log level and decreased in Androstar Premium by 2 to 3.5 log levels ( < 0.05). In semen samples from nine boars stored in the inoculated Androstar Premium extender containing half of the standard concentration of gentamicin, bacteria counts were below 10 CFU/mL. Likewise, half of the standard dose of apramycin and ampicillin was fully antimicrobially active and sperm quality was maintained. In conclusion, semen extenders with intrinsic antimicrobial activity allow a reduction in antibiotic use in pig insemination.
在牲畜精液稀释剂中使用抗生素可能会促进多重耐药性的发展和传播。由于猪人工授精精液剂量的储存温度相对较高（17°C），因此进行抗菌控制必不可少。本研究的目的，一是检查无抗生素稀释剂之间的抗菌能力是否存在差异，二是确定具有抗菌活性的稀释剂是否有可能减少抗生素的使用。将无抗生素精液稀释剂贝尔茨维尔解冻液（BTS）和Androstar Premium分别以10至10 CFU/mL的浓度接种从公猪射精中分离出的四种纯细菌菌株或其混合物，然后在17°C下储存144小时。需氧培养后的细菌计数在BTS中最多下降一个对数水平，在Androstar Premium中下降2至3.5个对数水平（<0.05）。在储存在含有一半标准庆大霉素浓度的接种Androstar Premium稀释剂中的九头公猪的精液样本中，细菌计数低于10 CFU/mL。同样，一半标准剂量的阿普拉霉素和氨苄青霉素具有完全的抗菌活性，并且精子质量得以维持。总之，具有内在抗菌活性的精液稀释剂可以减少猪人工授精中抗生素的使用。
