Ridgwell K, Roberts S J, Tanner M J, Anstee D J
Biochem J. 1983 Jul 1;213(1):267-9. doi: 10.1042/bj2130267.
Rhnull human erythrocytes lack all the antigens of the Rhesus blood-group system and are associated with mild chronic haemolytic anaemia. These erythrocytes have an abnormal shape and increased osmotic fragility. Labelling studies with the impermeant maleimide N-maleoylmethionine [35S]sulphone show that Rhnull erythrocytes lack two extracellular thiol-group-containing membrane components of apparent mol.wts. 32 000 and 34 000. Immunoprecipitation with mouse monoclonal antibody R6A (which reacts with all normal erythrocytes, but fails to react with Rhnull erythrocytes) specifically precipitates the 34 000-mol.wt. component from normal erythrocytes. Similar studies with human anti-Rh(D) serum shows that this antibody reacts with the 32 000-mol.wt. component. The results suggest that the R6A-binding polypeptide and the Rh(D) polypeptide may be involved in the maintenance of the shape and viability of the human erythrocyte.
Rh 阴性的人类红细胞缺乏恒河猴血型系统的所有抗原，并与轻度慢性溶血性贫血有关。这些红细胞形状异常，渗透脆性增加。用非渗透性马来酰亚胺 N-马来酰甲硫氨酸[35S]砜进行的标记研究表明，Rh 阴性红细胞缺乏两种细胞外表观分子量为 32000 和 34000 的含硫醇基团的膜成分。用小鼠单克隆抗体 R6A（与所有正常红细胞反应，但不与 Rh 阴性红细胞反应）进行免疫沉淀，可从正常红细胞中特异性沉淀出分子量为 34000 的成分。用人抗 Rh(D)血清进行的类似研究表明，该抗体与分子量为 32000 的成分反应。结果表明，R6A 结合多肽和 Rh(D)多肽可能参与维持人类红细胞的形状和活力。
