Avent N D, Ridgwell K, Mawby W J, Tanner M J, Anstee D J, Kumpel B
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
Biochem J. 1988 Dec 15;256(3):1043-6. doi: 10.1042/bj2561043.
The Rh D blood-group antigen forms part of a complex, involving several other polypeptides, that is deficient in the red cells of individuals who lack all the antigens of the Rh blood-group system (Rhnull red cells). These include components recognized by anti-(Rh D) antibodies and the murine monoclonal antibodies R6A and BRIC 125. We have carried out protein-sequence studies on the components immunoprecipitated by these antibodies. Anti-(Rh D) antibodies immunoprecipitate an Mr-30,000-32,000 polypeptide (the D30 polypeptide) and an Mr-45,000-100,000 glycoprotein (D50 polypeptide). Antibody R6A immunoprecipitates two glycoproteins of Mr 31,000-34,000 (R6A32 polypeptide) and Mr 35,000-52,000 (R6A45 polypeptide). The D30 and R6A32 polypeptides were found to have the same N-terminal amino acid sequences, showing that they are closely related proteins. The D50 polypeptide and the R6A45 polypeptide also had indistinguishable N-terminal amino acid sequences that differed from that of the D30 and R6A32 polypeptides. The putative N-terminal membrane-spanning segments of the two groups of proteins showed homology in their amino acid sequence, which may account for the association of each of the pairs of proteins during co-precipitation by the antibodies. Supplementary data related to the protein sequence have been deposited as Supplementary Publication SUP 50417 (6 pages) at the British Library Document Supply Centre, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1988) 249, 5.
Rh D血型抗原是一个复合体的一部分，该复合体还涉及其他几种多肽，在缺乏Rh血型系统所有抗原的个体（Rh阴性红细胞）的红细胞中不存在。这些成分包括抗（Rh D）抗体以及鼠单克隆抗体R6A和BRIC 125所识别的成分。我们对这些抗体免疫沉淀的成分进行了蛋白质序列研究。抗（Rh D）抗体免疫沉淀出一种分子量为30,000 - 32,000的多肽（D30多肽）和一种分子量为45,000 - 100,000的糖蛋白（D50多肽）。抗体R6A免疫沉淀出两种糖蛋白，分子量分别为31,000 - 34,000（R6A32多肽）和35,000 - 52,000（R6A45多肽）。发现D30和R6A32多肽具有相同的N端氨基酸序列，表明它们是密切相关的蛋白质。D50多肽和R6A45多肽也具有难以区分的N端氨基酸序列，且与D30和R6A32多肽的不同。两组蛋白质推测的N端跨膜片段在氨基酸序列上显示出同源性，这可能解释了在抗体共沉淀过程中每对蛋白质之间的关联。与蛋白质序列相关的补充数据已作为补充出版物SUP 50417（6页）存放在英国西约克郡韦瑟比波士顿温泉市的大英图书馆文献供应中心，邮编LS23 7BQ，可按《生物化学杂志》（1988年）249卷第5期所示条件从该中心获取复印件。
