白三烯C4的15-羟基化代谢产物的分离与鉴定

Isolation and characterization of 15-hydroxylated metabolites of leukotriene C4.

作者信息

Orning L, Hammarström S

出版信息

FEBS Lett. 1983 Mar 21;153(2):253-6. doi: 10.1016/0014-5793(83)80618-8.

Abstract

A polar metabolite of leukotriene C4 was formed by sequential conversions with soybean lipoxygenase I and liver peroxidase. The structure of this product was found to be 5(S), 15(S)-dihydroxy-6(R)-S-glutathionyl-7,9,13-trans-11-cis-eicosatetraenoic acid (15-hydroxy-delta 13-trans-leukotriene C3. The HPLC behaviour, the molar extinction coefficient and the biological activity of the metabolite are reported. Preliminary evidence suggests that this product is formed by mammalian tissues.

摘要

白三烯C4的一种极性代谢物是通过大豆脂氧合酶I和肝脏过氧化物酶的顺序转化形成的。发现该产物的结构为5(S),15(S)-二羟基-6(R)-S-谷胱甘肽基-7,9,13-反式-11-顺式-二十碳四烯酸（15-羟基-δ13-反式白三烯C3）。报道了该代谢物的高效液相色谱行为、摩尔消光系数和生物活性。初步证据表明该产物是由哺乳动物组织形成的。