Hernandez L M, Olivero I, Larriba G
FEBS Lett. 1983 Sep 19;161(2):190-4. doi: 10.1016/0014-5793(83)81005-9.
Accumulation and secretion of beta-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulation of active glucanases was observed. Following a shift to permissive conditions in the presence of cycloheximide a rise in the internal activity took place. The increase in total glucanase activity was partially due to the activation of an exo-glucanase that hydrolyzes PNPG. It is concluded that glucanases are synthesized in inactive precursor forms and are converted to the active forms in their secretory pathway.
通过使用在内质网水平受阻的酿酒酵母温度敏感型分泌突变体（sec 18-1），在体内研究了β-葡聚糖酶的积累和分泌。在限制温度下孵育时，未观察到活性葡聚糖酶的积累。在环己酰亚胺存在下转移到允许条件后，内部活性增加。总葡聚糖酶活性的增加部分归因于水解对硝基苯-β-D-葡萄糖苷（PNPG）的外切葡聚糖酶的激活。得出的结论是，葡聚糖酶以无活性的前体形式合成，并在其分泌途径中转化为活性形式。
