Schnorr K L, Pearson L D, Knisley K A, DeMartini J C
Int Arch Allergy Appl Immunol. 1983;72(3):239-42. doi: 10.1159/000234874.
The use of fluorescein isothiocyanate (FITC) as a cell marker in ovine lymphocyte circulation studies was investigated. The effects of the label on lymphocyte function were assessed by lymphocyte blastogenesis, cell-mediated cytotoxicity and normal lymphocyte transfer reactions. Labelling lymphocytes in 0.05 mg FITC/ml for 10 min at 37 degrees C was found to be optimum. Circulation kinetics of FITC-labelled efferent lymph lymphocytes were monitored as they disappeared from peripheral blood following intravenous infusion and reappeared in the efferent lymphatic duct of the popliteal lymph node. No functional changes were detected in lymphocytes labelled with the optimal regimen.
研究了异硫氰酸荧光素（FITC）作为细胞标记物在绵羊淋巴细胞循环研究中的应用。通过淋巴细胞增殖、细胞介导的细胞毒性和正常淋巴细胞转移反应评估标记对淋巴细胞功能的影响。发现在37℃下用0.05mg FITC/ml标记淋巴细胞10分钟是最佳的。监测FITC标记的输出淋巴淋巴细胞的循环动力学，观察其静脉注射后从外周血中消失并重新出现在腘淋巴结输出淋巴管中的情况。用最佳方案标记的淋巴细胞未检测到功能变化。
