Abernethy N J, Chin W, Lyons H, Hay J B
Cytometry. 1985 Sep;6(5):407-13. doi: 10.1002/cyto.990060504.
Substituted rhodamine isothiocyanate (XRITC) has been used to study lymphocyte migration in sheep. After being labeled in vitro with XRITC, lymphocytes appeared in the efferent lymph of single lymph nodes with the same kinetics as cells labeled with fluorescein isothiocyanate (FITC). The recovery of intravenously injected XRITC-labeled cells was followed in lymph for several days. The kinetics and recoveries were compared with data obtained using FITC, chromium-51, and indium-111. XRITC was found to be a suitable label and, using dual laser (argon and krypton) flow cytometry, it could be analyzed simultaneously with FITC. In addition, it was possible to relabel FITC-stained cells with XRITC after they were recovered in lymph. The migratory characteristics of such double-labeled cells were not different from single-labeled cells.
取代异硫氰酸罗丹明（XRITC）已被用于研究绵羊体内淋巴细胞的迁移。用XRITC在体外标记后，淋巴细胞出现在单个淋巴结的输出淋巴中，其动力学与用异硫氰酸荧光素（FITC）标记的细胞相同。静脉注射XRITC标记细胞后的回收情况在淋巴中持续观察了数天。将这些动力学和回收率与使用FITC、铬-51和铟-111获得的数据进行了比较。发现XRITC是一种合适的标记物，并且使用双激光（氩离子和氪离子）流式细胞仪，可以与FITC同时进行分析。此外，在淋巴中回收FITC染色的细胞后，可用XRITC对其重新标记。这种双重标记细胞的迁移特性与单一标记细胞没有差异。
