用于酶免疫测定和免疫组织化学染色的抗体及其片段的酶标记 - Suppr

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Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining.

作者信息

Ishikawa E, Imagawa M, Hashida S, Yoshitake S, Hamaguchi Y, Ueno T

出版信息

J Immunoassay. 1983;4(3):209-327. doi: 10.1080/15321818308057011.

DOI:10.1080/15321818308057011

PMID:6413534

Abstract

摘要

相似文献

Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining.用于酶免疫测定和免疫组织化学染色的抗体及其片段的酶标记

J Immunoassay. 1983;4(3):209-327. doi: 10.1080/15321818308057011.

Comparison of beta-D-galactosidase from Escherichia coli and horseradish peroxidase as labels of anti-human ferritin Fab' by sandwich enzyme immunoassay technique.采用夹心酶免疫测定技术比较大肠杆菌β-D-半乳糖苷酶和辣根过氧化物酶作为抗人铁蛋白Fab'标记物的研究

J Biochem. 1984 Sep;96(3):659-64. doi: 10.1093/oxfordjournals.jbchem.a134882.

[Comparative studies of peroxidase, alkaline phosphatase and beta-galactosidase as marker enzymes. Insertion into a colorimetric and fluorimetric enzyme immunoassay in IgG-conjugated form].[过氧化物酶、碱性磷酸酶和β-半乳糖苷酶作为标记酶的比较研究。以IgG偶联形式插入比色和荧光酶免疫测定中]

Z Med Lab Diagn. 1986;27(3):145-53.

Evaluation of beta-D-galactosidase from Escherichia coli and horseradish peroxidase as labels by sandwich enzyme immunoassay technique.采用夹心酶免疫测定技术评估来自大肠杆菌的β-D-半乳糖苷酶和辣根过氧化物酶作为标记物的情况。

Ann Clin Biochem. 1984 Jul;21 ( Pt 4):310-7. doi: 10.1177/000456328402100414.

A simple method for the conjugation of affinity-purified Fab' to horseradish peroxidase and beta-D-galactosidase from Escherichia coli.一种将亲和纯化的Fab'与来自大肠杆菌的辣根过氧化物酶和β-D-半乳糖苷酶偶联的简单方法。

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Sensitivity of steroid enzyme immunoassays. Comparison of alkaline phosphatase, beta-galactosidase and horseradish peroxidase as labels in a colorimetric assay system.

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Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase or beta-galactosidase?在比色和荧光酶免疫分析中，常用的标记酶辣根过氧化物酶、碱性磷酸酶或β-半乳糖苷酶，哪种能产生最佳结果？

J Immunol Methods. 1985 May 10;79(1):27-37. doi: 10.1016/0022-1759(85)90388-6.

Highly sensitive enzyme immunoassay of rabbit (anti-human IgG) IgG using human IgG-beta-D-galactosidase conjugate.使用人IgG-β-D-半乳糖苷酶偶联物对兔（抗人IgG）IgG进行高灵敏度酶免疫测定。

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Preparation of monomeric Fab'-horseradish peroxidase conjugate using thiol groups in the hinge and its evaluation in enzyme immunoassay and immunohistochemical staining.利用铰链区的巯基制备单体Fab'-辣根过氧化物酶偶联物及其在酶免疫测定和免疫组织化学染色中的评价。

Ann N Y Acad Sci. 1983;420:74-89. doi: 10.1111/j.1749-6632.1983.tb22191.x.

Conjugation of p-benzoquinone treated enzymes with antibodies and Fab fragments.

Immunochemistry. 1977 Nov-Dec;14(11-12):767-74. doi: 10.1016/0019-2791(77)90352-4.

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