Kato M, Kubo S, Naiki M
J Immunogenet. 1978 Feb;5(1):31-40. doi: 10.1111/j.1744-313x.1978.tb00628.x.
Blood group P antigen and Pk antigen were recently identified as glycosphingolipid globoside, GalNAc(beta, 1 leads to 3)Gal(alpha, 1 leads to 4)Gas(beta, 1 leads to 4)Glc(beta, 1 leads to 1)ceramide, and trihexosylceramide, Gal(alpha, 1 leads to 4)Gal(beta, 1 leads to 4)Gld(beta, 1 leads to 1)ceramide, respectively (Naiki & Marcus, 1974). The presence of antibodies to both antigens in the sera from rare p donors and antibodies to P antigen in the serum from a rare Pk donor were first demonstrated by the complement fixation test with the optimal antigen preparations containing each purified glycoplipid, lecithin and cholesterol. In the serum from a p donor, most of antibody population of anti-P was IgM and was cross-reacted to Forssman glycolipid, GalNAc(alpha, 1 leads to 3)GalNAc(beta, 1 leads to 3)Gal(alpha, 1 leads to 4)Gal(beta, 1 leads to 4)Glc(beta 1 leads to 1) ceramide, but the rest of the anti-P antibodies and most of anti-Pk antibodies were IgG and had marked specificities for globoside and trihexosylceramide, respectively.
血型P抗原和Pk抗原最近被鉴定为糖鞘脂神经节苷脂，即GalNAc(β,1→3)Gal(α,1→4)Glc(β,1→4)Glc(β,1→1)神经酰胺，以及三己糖神经酰胺，即Gal(α,1→4)Gal(β,1→4)Glc(β,1→1)神经酰胺（Naiki和Marcus，1974年）。通过使用含有每种纯化糖脂、卵磷脂和胆固醇的最佳抗原制剂进行补体结合试验，首次证明了罕见p供体血清中存在针对这两种抗原的抗体，以及罕见Pk供体血清中存在针对P抗原的抗体。在p供体的血清中，抗P抗体的大多数群体是IgM，并且与福斯曼糖脂GalNAc(α,1→3)GalNAc(β,1→3)Gal(α,1→4)Gal(β,1→4)Glc(β1→1)神经酰胺发生交叉反应，但其余的抗P抗体和大多数抗Pk抗体是IgG，并且分别对神经节苷脂和三己糖神经酰胺具有明显的特异性。
