Uemura K, Yuzawa-Watanabe M, Kitazawa N, Taketomi T
J Biochem. 1980 Jun;87(6):1641-8. doi: 10.1093/oxfordjournals.jbchem.a132908.
Antibodies against each of three neutral glycosphingolipids, Forssman glycolipid, globoside I, and asialo GM2, were elicited in rabbits. The specificity of these antibodies was studied by liposome agglutination and liposomal membrane immune-damage assays using liposomes prepared with each glycolipid hapten. Spectrophotometric measurement of liposome agglutination was found to be effective for detecting IgG antibodies, whereas complement-dependent immune-damage assay favored the expression of IgM antibodies. Three glycosphingolipids having the same non-reducing terminal N-acetylgalactosamine residues were discriminated with IgG antibodies, while IgM antibodies showed cross-reactivity. Specifically purified anti-Forssman and anti-asialo GM2 antibodies reacted only with Forssman glycolipid and asialo Gm2, respectively, but purified anti-globoside I antibodies cross-reacted with asialo GM2.
在兔子体内诱导产生了针对三种中性糖鞘脂（福斯曼糖脂、红细胞糖苷脂I和脱唾液酸GM2）的抗体。通过脂质体凝集和脂质体膜免疫损伤试验，利用每种糖脂半抗原制备的脂质体，研究了这些抗体的特异性。发现分光光度法测量脂质体凝集对于检测IgG抗体有效，而补体依赖性免疫损伤试验有利于IgM抗体的表达。具有相同非还原末端N-乙酰半乳糖胺残基的三种糖鞘脂可以用IgG抗体区分，而IgM抗体表现出交叉反应性。特异性纯化的抗福斯曼和抗脱唾液酸GM2抗体分别仅与福斯曼糖脂和脱唾液酸GM2反应，但纯化的抗红细胞糖苷脂I抗体与脱唾液酸GM2发生交叉反应。
