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噬菌体SPO1基因27:定位与核苷酸序列

Bacteriophage SPO1 gene 27: location and nucleotide sequence.

作者信息

Costanzo M, Hannett N, Brzustowicz L, Pero J

出版信息

J Virol. 1983 Nov;48(2):555-60. doi: 10.1128/JVI.48.2.555-560.1983.

DOI:10.1128/JVI.48.2.555-560.1983
PMID:6413701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255381/
Abstract

Bacteriophage SPO1 gene 27, whose product is required for late gene transcription and DNA replication, has been cloned in Escherichia coli, and its complete nucleotide sequence has been determined. We infer that the product of gene 27 is a highly basic 17,518-dalton protein of 155 amino acids. The gene for this regulatory protein is transcribed from two promoters: an early promoter situated before the adjacent upstream gene 28 and a middle promoter located between genes 28 and 27.

摘要

噬菌体SPO1的基因27,其产物是晚期基因转录和DNA复制所必需的,已在大肠杆菌中克隆,并确定了其完整的核苷酸序列。我们推断基因27的产物是一种由155个氨基酸组成的高度碱性的17518道尔顿蛋白质。这种调节蛋白的基因从两个启动子转录:一个早期启动子位于相邻的上游基因28之前,一个中期启动子位于基因28和27之间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/255381/517c7427f2e7/jvirol00140-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/255381/521e8f1bb658/jvirol00140-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/255381/517c7427f2e7/jvirol00140-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/255381/521e8f1bb658/jvirol00140-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/255381/517c7427f2e7/jvirol00140-0231-a.jpg

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1
Bacteriophage SPO1 gene 27: location and nucleotide sequence.噬菌体SPO1基因27:定位与核苷酸序列
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引用本文的文献

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Regulation of bacteriophage P2 late-gene expression: the ogr gene.噬菌体P2晚期基因表达的调控:ogr基因

本文引用的文献

1
Transcriptional regulation of bacteriophage SPO1 protein synthesis in vivo and in vitro.噬菌体SPO1蛋白合成在体内和体外的转录调控
J Virol. 1982 Jun;42(3):951-62. doi: 10.1128/JVI.42.3.951-962.1982.
2
SP01 gene 27 is required for viral late transcription.病毒晚期转录需要SP01基因27。
J Virol. 1982 Feb;41(2):715-20. doi: 10.1128/JVI.41.2.715-720.1982.
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Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene.革兰氏阳性金黄色葡萄球菌β-内酰胺酶基因核糖体结合位点序列的独特特征。
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TF1, the bacteriophage SPO1-encoded type II DNA-binding protein, is essential for viral multiplication.TF1是噬菌体SPO1编码的II型DNA结合蛋白,对病毒繁殖至关重要。
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Structure of a Bacillus subtilis bacteriophage SPO1 gene encoding RNA polymerase sigma factor.枯草芽孢杆菌噬菌体SPO1编码RNA聚合酶σ因子的基因结构。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1236-40. doi: 10.1073/pnas.80.5.1236.
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A high speed, high capacity homology matrix: zooming through SV40 and polyoma.一种高速、高容量的同源性矩阵:快速分析猿猴病毒40和多瘤病毒
Nucleic Acids Res. 1982 Aug 11;10(15):4765-82. doi: 10.1093/nar/10.15.4765.
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Bacteriophage T4 gene 45. Sequences of the structural gene and its protein product.噬菌体T4基因45。结构基因及其蛋白质产物的序列。
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8
Conserved nucleotide sequences in temporally controlled bacteriophage promoters.时间控制型噬菌体启动子中的保守核苷酸序列。
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A transcriptional map of the bacteriophage SPO1 genome. III. A region of early and middle promoters (the gene 28 region).噬菌体SPO1基因组的转录图谱。III. 早期和中期启动子区域(基因28区域)。
Virology. 1981 Jul 30;112(2):572-88. doi: 10.1016/0042-6822(81)90303-2.
10
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.