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荧光假单胞菌和恶臭假单胞菌中的分子DNA-DNA杂交

[Molecular DNA-DNA hybridization in Pseudomonas fluorescens and Pseudomonas putida].

作者信息

Kiprianova E A, Levanova G F, Shvetsov Iu P, Garagulia A D

出版信息

Mikrobiologiia. 1983 May-Jun;52(3):360-4.

PMID:6413827
Abstract

Taxonomic analysis was carried out and DNA-DNA homology studied in cultures of subgroups formed by numerical classification of 124 Pseudomonas fluorescens and P. putida strains. The GC content in the DNA of strains belonging to P. fluorescens subgroups varied from 61.2 to 64.5%, and their DNA-DNA homology with the type strain P. fluorescens ATCC 13525 was 24 to 83%. The lowest genome relatedness with the type culture was found in biotype C of P. fluorescens and P. aureofaciens strains. The GC content in the DNA of strains belonging to two subgroups formed by numerical classification of P. putida varied from 63.8 to 65.0%, and the homology of their DNA with the DNA of the type strain of P. fluorescens was 0 to 10% (for strains of the first and second subgroups, respectively).

摘要

对由124株荧光假单胞菌和恶臭假单胞菌菌株进行数值分类所形成的亚群培养物进行了分类分析,并研究了DNA-DNA同源性。荧光假单胞菌亚群菌株的DNA中GC含量在61.2%至64.5%之间,它们与荧光假单胞菌模式菌株ATCC 13525的DNA-DNA同源性为24%至83%。在荧光假单胞菌生物型C和金黄色假单胞菌菌株中发现与模式培养物的基因组相关性最低。由恶臭假单胞菌数值分类形成的两个亚群菌株的DNA中GC含量在63.8%至65.0%之间,它们的DNA与荧光假单胞菌模式菌株的DNA同源性分别为0%至10%(分别针对第一和第二亚群的菌株)。

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