Re-identification of strains deposited as , and in GenBank based on whole genome sequences.

The taxonomic classification of species has been revised and updated several times. This study utilized average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) cutoff values of 95 and 70 %, respectively, to re-identify the species of strains deposited in GenBank as , and . Of the 264 deposited strains, 259 were correctly identified as , but the remaining five were not. All 28 deposited strains had been incorrectly identified as . Four of these strains were re-identified, including two as and one each as and , but the remaining 24 could not be re-identified. Similarly, all 35 deposited strains had been incorrectly identified as . Nineteen of these strains were re-identified, including 12 as , four as and one each as , and . These results strongly suggest that bacteria should be identified using ANI and dDDH analyses based on whole genome sequencing when species are initially deposited in GenBank/DDBJ/EMBL databases.