Starr J I, Mako M E, Juhn D, Rubenstein A H
J Lab Clin Med. 1978 Apr;91(4):683-92.
The variation in cross-reactivity of human and porcine insulins and proinsulins was determined with a number of insulin antisera. The relative immunoreactivity of insulin and proinsulin with these antisera ranged from a ratio of 1.08 to 5.7 on a molar basis. The displacement curves of human insulin and proinsulin were not parallel, and the relationship of porcine and human proinsulin varied with different antisera. Sera with varying concentrations of PLM (proinsulin and its intermediate components) and serum PLM and insulin fractions (separated by gel filtration) were measured in two assays using the antisera which reacted most differently with human insulin and proinsulin standards. With the use of these two antisera, measurement of serum PLM against a human insulin standard gave different results, which were only partially corrected by reading the values from a human proinsulin standard. The magnitude of the serum IRI differences with these antisera was related to the proinsulin/insulin ratio in each sample. These results indicate the necessity for each laboratory to critically evaluate the reaction of their insulin antiserum with human proinsulin. In addition, measurement of serum PLM in terms of a insulin standard will give different results depending upon the particular antiserum used.
使用多种胰岛素抗血清测定了人和猪胰岛素及胰岛素原交叉反应性的变化。胰岛素和胰岛素原与这些抗血清的相对免疫反应性在摩尔基础上的比值范围为1.08至5.7。人胰岛素和胰岛素原的置换曲线不平行，猪和人胰岛素原的关系因不同抗血清而异。在两项检测中，使用与人胰岛素和胰岛素原标准品反应差异最大的抗血清，测定了不同浓度的PLM（胰岛素原及其中间成分）血清以及血清PLM和胰岛素组分（通过凝胶过滤分离）。使用这两种抗血清，以人胰岛素标准品测定血清PLM得出了不同结果，通过读取人胰岛素原标准品的值仅能部分校正这些结果。这些抗血清导致的血清IRI差异大小与每个样品中的胰岛素原/胰岛素比值有关。这些结果表明每个实验室都有必要严格评估其胰岛素抗血清与人胰岛素原的反应。此外，根据所用的特定抗血清，以胰岛素标准品测定血清PLM会得出不同结果。
