Vostroknutova G N, Kaprel'iants A S, Svetlichnyĭ V A, El'-Registan G I, Shevtsov V V
Prikl Biokhim Mikrobiol. 1983 Jul-Aug;19(4):547-51.
The effect of a preparation obtained by butanol extraction of the culture fluid of B. cereus and Ps. carboxydoflava and previously termed an autoregulatory factor d1 on the respiration chain within intact bacterial cells and isolated membranes was investigated. In comparable concentrations this factor d1 inhibits the endogenous respiration of B. cereus and M. lysodeikticus as well as oxidase and dehydrogenase activities of isolated membranes from these bacteria and E. coli. The factor-induced decrease of the cell respiration rate is independent from disorders of the cell permeability osmotic barrier for respiration substrates. The factor d1 shows a higher effect at acidic pH values. It is concluded that the above preparation has membrane-active properties. It is also suggested that in the bacterial cell its target is the inner surface of the cytoplasmic membrane.
研究了通过对蜡样芽孢杆菌和嗜羧基黄杆菌培养液进行丁醇萃取而获得的一种制剂（先前称为自调节因子d1）对完整细菌细胞和分离膜内呼吸链的影响。在相当的浓度下，该因子d1抑制蜡样芽孢杆菌和溶壁微球菌的内源性呼吸以及这些细菌和大肠杆菌分离膜的氧化酶和脱氢酶活性。该因子引起的细胞呼吸速率降低与呼吸底物的细胞通透性渗透屏障紊乱无关。因子d1在酸性pH值下表现出更高的效应。得出的结论是，上述制剂具有膜活性特性。还表明，在细菌细胞中其作用靶点是细胞质膜的内表面。
