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采用气固色谱法测定呼吸气体(二氧化碳、氧气、氩气和氮气)。

Determination of respiratory gases (CO2, O2, Ar and N2) with gas solid chromatography.

作者信息

Johansson U B

出版信息

Scand J Clin Lab Invest. 1983 Feb;43(1):91-4. doi: 10.1080/00365518309168227.

DOI:10.1080/00365518309168227
PMID:6414074
Abstract

A method was developed for gas chromatographic separation of respiratory gases (CO2, O2, Ar and N2). Oxygen was catalystically reduced to H2O by palladium, with H2 as carrier gas. CO2 and H2O were separated on a Porapack Q column and detected separately. After absorption of CO2 and H2O, Ar and N2 were separated by a molecular sieve column and recorded by the reference cell. Comparison with the Scholander technique gave the correlation coefficient 0.997 for CO2 and 0.992 for O2 respectively. All values were close to the line of identity. We found it necessary to calibrate with a known gas mixture before each set of analyses for accurate determinations.

摘要

开发了一种用于气相色谱分离呼吸气体(二氧化碳、氧气、氩气和氮气)的方法。氧气在钯的催化作用下被还原为水,氢气作为载气。二氧化碳和水在Porapack Q柱上分离并分别检测。吸收二氧化碳和水后,氩气和氮气通过分子筛柱分离并用参比池记录。与Scholander技术比较,二氧化碳的相关系数分别为0.997,氧气为0.992。所有值均接近恒等线。我们发现每次分析前用已知气体混合物进行校准对于准确测定是必要的。

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Scand J Clin Lab Invest. 1983 Feb;43(1):91-4. doi: 10.1080/00365518309168227.
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