Wilderspin A F, Green N M
Anal Biochem. 1983 Jul 15;132(2):449-55. doi: 10.1016/0003-2697(83)90032-5.
The reversible formation of dithiourethanes from fluorescein isothiocyanate and mercaptoethanol or mercaptoethylamine has been studied. It is shown that it is possible to use the reaction as the basis for a spectrophotometric titration of a number of isothiocyanates with mercaptoethanol.
研究了异硫氰酸荧光素与巯基乙醇或巯基乙胺可逆形成二硫代氨基甲酸酯的过程。结果表明，该反应可作为用巯基乙醇对多种异硫氰酸酯进行分光光度滴定的基础。
