荧光串联藻胆蛋白缀合物。通过能量转移实现发射波长的移动。
Fluorescent tandem phycobiliprotein conjugates. Emission wavelength shifting by energy transfer.
作者信息
Glazer A N, Stryer L
出版信息
Biophys J. 1983 Sep;43(3):383-6. doi: 10.1016/S0006-3495(83)84361-6.
Abstract
A fluorescent tandem phycobiliprotein conjugate with a large Stokes shift was prepared by the covalent attachment of phycoerythrin to allophycocyanin. The efficiency of energy transfer from phycoerythrin to allophycocyanin in this disulfide-linked conjugate was 90%. A distinctive feature of this phycocyanin conjugate is the wide separation between the intense absorption maximum of phycoerythrin (epsilon = 2.4 x 10(6) cm-1 M-1 at 545 nm) and the fluorescence emission maximum of allophycocyanin (660 nm). Energy transfer from a donor to a covalently attached acceptor can be used to adjust the magnitude of the Stokes shift. Tandem phycobiliprotein conjugates can be used to advantage in fluorescence-activated cell sorting, fluorescence microscopy, and fluorescence immunoassay analyses.
摘要
通过将藻红蛋白共价连接到别藻蓝蛋白上，制备了一种具有大斯托克斯位移的荧光串联藻胆蛋白共轭物。在这种二硫键连接的共轭物中，从藻红蛋白到别藻蓝蛋白的能量转移效率为90%。这种藻蓝蛋白共轭物的一个显著特点是藻红蛋白的强吸收最大值（在545nm处ε = 2.4×10⁶ cm⁻¹ M⁻¹）与别藻蓝蛋白的荧光发射最大值（660nm）之间有很大的间隔。从供体到共价连接受体的能量转移可用于调节斯托克斯位移的大小。串联藻胆蛋白共轭物可有效地用于荧光激活细胞分选、荧光显微镜检查和荧光免疫分析。
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