Liu C K, Schmied R, Schreiber C, Rosen A, Qian G X, Waxman S
Exp Hematol. 1983 Sep;11(8):738-46.
We have assayed glycosyltransferase activities during the granulocytic and macrophage-like differentiation of human promyelocytic leukemia (HL-60) cells. Functional granulocytic differentiation was assayed by the decarboxylation of 2-deoxyglucose in addition to nitroblue tetrazolium reduction. Dimethylsulfoxide (DMSO) treated HL-60 cells, induced to granulocytic differentiation, had higher 2-deoxy-glucose decarboxylation activity, and contained less sialyltransferase (ST), more fucosyltransferase (FT), and more N-acetylglucosaminyltransferase (NGT) activities than untreated cells. HL-60 cells treated with another granulocytic differentiator, retinoic acid, also had higher 2-deoxyglucose decarboxylation activity, and contained less ST, more FT, and more NGT activities than untreated cells. In contrast, cells treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) reported to differentiate HL-60 to macrophage-like cells, but did not show an increased level of 2-deoxyglucose decarboxylation activity, but contained more galactosyltransferase (GT) and FT activities as compared to untreated cells. These findings suggest that the alterations of glycosyltransferase levels during the differentiation of precursor cells may not depend upon different inducers, but are characteristic of the phenotypic expression of the mature cell type.
我们已经检测了人早幼粒细胞白血病（HL-60）细胞在粒细胞和巨噬细胞样分化过程中的糖基转移酶活性。除了硝基蓝四氮唑还原试验外，还通过2-脱氧葡萄糖的脱羧反应来检测功能性粒细胞分化。经二甲基亚砜（DMSO）处理诱导粒细胞分化的HL-60细胞，其2-脱氧葡萄糖脱羧活性更高，与未处理细胞相比，唾液酸转移酶（ST）活性更低，岩藻糖基转移酶（FT）和N-乙酰葡糖胺基转移酶（NGT）活性更高。用另一种粒细胞分化诱导剂维甲酸处理的HL-60细胞，同样具有更高的2-脱氧葡萄糖脱羧活性，与未处理细胞相比，ST活性更低，FT和NGT活性更高。相比之下，用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理的细胞，据报道可使HL-60细胞分化为巨噬细胞样细胞，但2-脱氧葡萄糖脱羧活性并未升高，不过与未处理细胞相比，其半乳糖基转移酶（GT）和FT活性更高。这些发现表明，前体细胞分化过程中糖基转移酶水平的改变可能不取决于不同的诱导剂，而是成熟细胞类型表型表达的特征。
