Zucker R M, Whittington K, Price B J
Cytometry. 1983 May;3(6):414-8. doi: 10.1002/cyto.990030605.
HL-60 promyelocytic leukemic cells can differentiate into more mature myeloid cells with the addition of dimethylsulfoxide, butyric acid or retinoic acid and can differentiate into macrophages with the addition of phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA). After the addition of an inducer, the HL-60 cell volume shows a daily decrease while the cell number increases at a rate similar to the untreated control cells. Flow cytometry measurements show an increase in G1 cells and a decrease in S cells after day 1. Since the generation time is constant, the data suggest that the length of time spent in the different cell cycle stages has changed during differentiation. Within 3 hours after the addition of TPA to HL-60 cells, selective adhesion of G1 cells occurs. Smaller sized cells are recovered from the flask bottom and larger sized cells are recovered from the supernate. Flow cytometric analysis reveals a G1 and S block in cells obtained from both the supernatant and from the flask bottom. After 1 day of TPA incubation, there is preferential adhesion of G1 and G2 cells with the nonadherent cells being primarily in the S and G2 cell cycle stages and undergoing a cell cycle traverse.
HL-60早幼粒细胞白血病细胞在添加二甲基亚砜、丁酸或视黄酸后可分化为更成熟的髓样细胞，在添加佛波酯12-O-十四酰佛波醇-13-乙酸酯（TPA）后可分化为巨噬细胞。添加诱导剂后，HL-60细胞体积每日减小，而细胞数量以与未处理对照细胞相似的速率增加。流式细胞术测量显示，第1天后G1期细胞增加，S期细胞减少。由于细胞周期时间恒定，数据表明在分化过程中，细胞在不同细胞周期阶段所花费的时间长度发生了变化。在向HL-60细胞添加TPA后3小时内，G1期细胞发生选择性黏附。较小的细胞从培养瓶底部回收，较大的细胞从上清液中回收。流式细胞术分析显示，从上清液和培养瓶底部获得的细胞中均存在G1期和S期阻滞。TPA孵育1天后，G1期和G2期细胞优先黏附，未黏附的细胞主要处于S期和G2期细胞周期阶段并经历细胞周期转换。
