Chang T S, Zirkin B R
J Exp Zool. 1978 May;204(2):283-9. doi: 10.1002/jez.1402040216.
Rabbit sperm nuclei decondensed when incubated with dithiothreitol and Triton X-100. Protamine isolated from these nuclei during the course of treatment exhibited marked and progressive degradation. Timed interval experiments revealed that the degradation of protamine significantly preceded sperm nuclear decondensation and reached its maximum extent before decondensation was completed. Studies with protease inhibitors demonstrated that proteolysis was involved in both nuclear decondensation and protamine degradation.
兔精子细胞核在与二硫苏糖醇和 Triton X - 100 一起孵育时会解聚。在处理过程中从这些细胞核中分离出的鱼精蛋白表现出明显且逐步的降解。定时间隔实验表明，鱼精蛋白的降解显著先于精子细胞核解聚，并在解聚完成前达到最大程度。蛋白酶抑制剂研究表明，蛋白水解参与了细胞核解聚和鱼精蛋白降解过程。
