Zirkin B R, Boison A, Heston W D, Coffey D S
J Exp Zool. 1976 Aug;197(2):283-8. doi: 10.1002/jez.1401970209.
The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X-100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions for DNA synthesis are released. By contrast, similar thiol treatment of isolated rat liver nuclei fails to release DNA template restrictions. These results suggest a significant difference in the control of template activity in mammalian sperm and somatic cell nuclei.
比较了二硫键还原对兔精子和大鼠肝细胞核DNA模板活性的影响。在室温下用5 mM二硫苏糖醇和1% Triton X-100处理兔精子90分钟后，精子核解聚，DNA合成的核模板限制被解除。相比之下，对分离的大鼠肝细胞核进行类似的硫醇处理未能解除DNA模板限制。这些结果表明哺乳动物精子和体细胞细胞核在模板活性控制方面存在显著差异。
